DNA-PROTECTIVE EFFECT OF POLYAMINES AS THE FACTOR OF ESCHERICHIA COLI LEVOFLOXACIN

Authors

  • Лариса Юрьевна Нестерова Institute of Ecology and Genetics of Microorganisms UB RAS
  • Анна Викторовна Ахова Institute of Ecology and Genetics of Microorganisms UB RAS
  • Михаил Сергеевич Шумков Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences
  • Александр Георгиевич Ткаченко Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State University

Keywords:

antibiotic resistance, polyamines, fluoroquinolones, dna damage, reactive oxygen species

Abstract

Effect of natural polyamines on susceptibility of Escherichia coli clinical isolates to fluoroquinolone antibiotic levofloxacin was studied. Objects: E. coli clinical isolates of different fluoroquinolone resistance level (sensitive, intermediate and resistant). Methods: estimation of antibiotic minimal inhibitory concentration by the standard 2fold dilution antimicrobial susceptibility test; registration of DNA damage by agarose gel electrophoresis of pDNA samples extracted out of E. coli cells exposed to antibiotics and polyamines; determination of hydroxyl radicals with 3'-(hydroxyphenyl) fluorescein cell staining followed by fluorescent detection with microplate reader. We found that putrescine and spermidine, but not cadaverine, increased significantly the resistance of E. coli clinical isolates with different susceptibility to levofloxacin in a concentration-dependent manner. Antibiotic activity of levofloxacin was accompanied by promotion of reactive oxygen species formation and DNA damage in bacterial cells. When added to antibiotic-treated cells, polyamines putrescine and spermidine reduced the in-tracellular production of reactive oxygen species and prevented DNA damage. Thereby, it was found that poly-amines putrescine and spermidine decrease the susceptibility of E. coli isolates to fluoroquinolone antibiotic levofloxacin through, in particular, their antioxidant activity.

Author Biographies

  • Лариса Юрьевна Нестерова, Institute of Ecology and Genetics of Microorganisms UB RAS
    Senior scientist of the laboratory of microorganisms adaptation
  • Анна Викторовна Ахова, Institute of Ecology and Genetics of Microorganisms UB RAS
    Candidate of biology, research assistant of the laboratory of microorganisms adaptation
  • Михаил Сергеевич Шумков, Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences
    Candidate of biology, research assistant of the laboratory of biochemistry of stresses in microorganisms
  • Александр Георгиевич Ткаченко, Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State University
    Head of the laboratory of microorganisms adaptation$Professor of the Department of Microbiology and immunology

References

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Agostinelli Е. Polyamines in biological systems // Amino Acids. 2010. Vol. 38, № 2. P. 351-352.

Bloomfield VA. DNA condensation // Current Opinion in Structural Biology. 1996. Vol. 6, № 3. P. 334-341.

Das K.C., Misra H.P. Hydroxyl radical scavenging and singlet oxygen quenching properties of polyamines // Molecular and Cellular Biochemistry. 2004. Vol. 262, № 1-2. P. 127-33.

Douki Т., Bretonniere Y, Cadet J. Protection against radiation-induced degradation of DNA bases by polyamines // Radiation Research. 2000. Vol. 153. P. 29-35.

Drlica K, Malik M., Kerns R.J., Zhao X. Quinolone-mediated bacterial death // Antimicrobial Agents and Chemotherapy. 2008. Vol. 52, № 2. P. 85-92.

Gogoi M., Datey A., Wilson K.T., Chakravortty D. Dual role of arginine metabolism in establishing pathogenesis // Current Opinion in Microbiology. 2016. Vol. 29. P. 43-48.

Kern W.V., Oethinger M., Jellen-Ritter A., Levy S.V. Non-Target Gene Mutations in the Development of Fluoroquinolone Resistance in Escherichia coli II Antimicrobial Agents and Chemotherapy. 2000. Vol. 44, №4. P. 814-820.

Kohanski M.A., Dwyer D.J., Hayete B., Lawrence C.A., Collins J.J. A common mechanism of cellular death induced by bactericidal antibiotics // Cell. 2007. Vol. 130, № 5. P. 797-810.

Miller-Fleming L., Olin-Sandoval V., Campbell K, Raiser M. Remaining mysteries of molecular biology: the role of polyamines in the cell // Journal of Molecular Biology. 2015. Vol. 427, № 21. P. 3389-3406.

Ruiz-Chica J., Medina M.A., Sánchez-Jiménez F., Ramírez F.J. Fourier transform Raman study of the structural specificities on the interaction between DNA and biogenic polyamines // Biophysical Journal. 2001. Vol. 80, № 1. P. 443-454.

Setsukinai K, Urano Y., Kakinuma K, Majima H.J., Nagano T. Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species // The Journal of Biological Chemistry. 2003. Vol. 278, №5. P. 3170-3175.

Sy D., Hugot S., Savoye C., Ruiz S., Charlier M., Spotheim-Maurizot M. Radioprotection of DNA by spermine: a molecular modelling approach // International Journal of Radiation Biology. 1999. Vol. 75, № 8. P. 953-961.

Tkachenko A., Akhova A., Shumkov M., Nesterova L. Polyamines reduce oxidative stress in Escherichia coli cells exposed to bactericidal antibiotics // Research in Microbiology. 2012. Vol. 163, № 2. P. 83-91.

Tkachenko A., Nesterova L., Pshenichnov M. The role of the natural polyamine putrescine in defense against oxidative stress in Escherichia coli II Archives of Microbiology. 2001. Vol. 176. P. 155-157.

References

Agostinelli E. Polyamines in biological systems. Amino Acids. V. 38, N 2 (2010): pp. 351-352.

Bloomfield V.A. DNA condensation. Current Opinion in Structural Biology V. 6, N 3 (1996): pp. 334-341.

Das K.C., Misra H.P. Hydroxyl radical scavenging and singlet oxygen quenching properties of polyamines. Molecular and Cellular Biochemistry. V. 262, N 1-2 (2004): pp. 127-33.

Douki T., Bretonniere Y., Cadet J. Protection against radiation-induced degradation of DNA bases by polyamines. Radiation Research. V. 153 (2000): pp. 29-35.

Drlica K., Malik M., Kerns R.J., Zhao X. Quinolone-mediated bacterial death. Antimicrobial Agents and Chemotherapy. V. 52, N 2 (2008): pp. 85-92.

Gogoi M., Datey A., Wilson K.T., Chakravortty D. Dual role of arginine metabolism in establishing pathogenesis. Current Opinion in Microbiology.V. 29 (2016): pp. 43-48.

Kern W.V., Oethinger M., Jellen-Ritter A., Levy S.V. Non-Target Gene Mutations in the Development of Fluoroquinolone Resistance in Escherichia coli. Antimicrobial Agents and Chemotherapy. V. 44, N4 (2000): pp. 814-820.

Kohanski M.A., Dwyer D.J., Hayete B., Lawrence C.A., Collins J.J. A common mechanism of cellular death induced by bactericidal antibiotics. Cell. V. 130, N 5 (2007): pp. 797-810.

Miller-Fleming L., Olin-Sandoval V., Campbell K., Raiser M. Remaining mysteries of molecular biology: the role of polyamines in the cell. Journal of Molecular Biology. V. 427, N 21 (2015): pp. 3389-3406.

Nesterova L.Yu., Tkachenko A.G. [The role of general stress adaptation factors in the development of Escherichia coli fluoroquinolone resistance] Vestnik Permskogo Universisteta. Ser.Biologija. Iss. 1 (2010): pp. 21-26. (In Russ.).

Ruiz-Chica J., Medina M.A., Sánchez-Jiménez F., Ramírez F.J. Fourier transform Raman study of the structural specificities on the interaction between DNA and biogenic polyamines. Biophysical Journal. V. 80, N 1 (2001): pp. 443-454.

Setsukinai K., Urano Y., Kakinuma K., Majima H.J., Nagano T. Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species. The Jor-nal of Biological Chemistry. V. 278, N 5. (2003): pp. 3170-3175.

Sy D., Hugot S., Savoye C., Ruiz S., Charlier M., Spotheim-Maurizot M. Radioprotection of DNA by spermine: a molecular modelling approach. International Journal of Radiation Biology. V. 75, N8, (1999): pp. 953-961.

Tkachenko A., Akhova A., Shumkov M., Nesterova L. Polyamines reduce oxidative stress in Escherichia coli cells exposed to bactericidal antibiotics. Research in Microbiology. V. 163, N 2 (2012): pp. 83-91.

Tkachenko A., Nesterova L., Pshenichnov M. The role of the natural polyamine putrescine in defense against oxidative stress in Escherichia coli. Archives of Microbiology. V. 176 (2001): pp. 155-157.

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Published

2018-10-22

Issue

No. 1 (2016): Bulletin of Perm University. Biology

Section

Микробиология

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How to Cite

DNA-PROTECTIVE EFFECT OF POLYAMINES AS THE FACTOR OF ESCHERICHIA COLI LEVOFLOXACIN. (2018). Bulletin of Perm University. Biology, 1, 54-59. https://press.psu.ru/index.php/bio/article/view/1788

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