MICROBIAL COMMUNITY OF THE TECHNOGENICALLY POLLUTED SOIL OF SALT-MINING REGION (BEREZNIKI)
Keywords:
denaturing gradient gel electrophoresis, 16S rRNA gene, nonculturable bacteria, ortho-phthalate, biphenyl, HalomonasAbstract
With the use of molecular-genetic methods a study of the structure of microbial community from the soil located near the BKPRU-3 salt separator of PAO Uralkali (Berezniki, Perm Territory) enterprise was carried out. The presence of 16S rRNA bacterial genes in the amount of 6,6 х 1011 (±8,87 х 107) copies of the gene per gram of soil and the Gram-negative bacteria dominance were detected by the real-time-PCR of the 16S rRNA gene fragments in the total DNA of the soil sample. The DGGE-analysis of 16S rDNA genes, amplified from total DNA, revealed that the bacterial communities of enrichment cultures, obtained on biphenyl and ortAo-phthalate, differed significantly from each other, as well as from the original community of the soil sample, both in composition and in the presence of dominant species. It was established that gram-negative bacteria of Gammaproteobacteria and Acidobacteria classes dominated in the derived enrichment cultures. By the method of DGGE-analysis "uncultivated" bacteria of the genus Halomonas were detected in the investigated microbial community.References
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