NK cells pretreated with estriol and commensal flora cells regulate the maturation of Treg and Th17 from CD4+ lymphocytes in multiple sclerosis patients and healthy donors

Abstract

Multiple sclerosis (MS) is an inflammatory chronic disease of the central nervous system, one of the pathogenetic mechanisms of which is an immune-mediated attack to the myelin sheath of nerve axons under the control of autoreactive T cells. NK cells, one of subtypes of ILC cells, have the ability to regulate autoimmune mechanisms through the production of cytokines or cytotoxic effects directly against effector cells, such as autoreactive T cells or antigen-presenting cells. At the same time, they can also lyse microglial, astrocyte, and oligodendrocyte cells, indicating a dual function of NK cells in MS. The influence of the pregnancy hormone estriol (E3) and commensal microflora on the ability of NK cells to modulate the level of T regulatory (Treg) and IL-17-producing (Th17) lymphocytes from patients with multiple sclerosis (MS) in comparison with healthy donors was studied. To activate NK cells, Ecsherichia coli K12 and Lactobacillus plantarum 8R-A3 strains were used. The phenotype of Treg and Th17 cells was assessed by flow cytometry using monoclonal antibody staining. It was found that NK cells incubated with E3 and E. coli stimulate the formation of Tregs, and pretreatment of NK cells with L. plantarum inhibits the formation of Th17 from CD4+ lymphocytes. In general, regulatory lymphocytes from MS patients turned out to be more sensitive to the influence of NK cells than cells from healthy donors.