Molecular genetic characteristics of pathogenicity factors of genus Acinetobacter representatives isolated in medical organizations of Rostov-on-Don

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Published: Jul 7, 2025
DOI: https://doi.org/10.17072/1994-9952-2025-2-185-195
Keywords:
A. baumannii A. calcoaceticus A. junii genome-wide sequencing pathogenicity factors

Main Article Content

Elena N. Gudueva
Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor, Rostov-on-Don, Russia
Victoria V. Agafonova
Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor, Rostov-on-Don, Russia
Natalia E. Gayevskaya
Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor, Rostov-on-Don, Russia
Valentina A. Lychman
Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor, Rostov-on-Don, Russia
Alexandr Y. Karnayxov
Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor, Rostov-on-Don, Russia
Olga S. Chemisova
Don State Technical University, DSTU, Rostov-on-Don, Russia

Abstract

The most common causative agent of nosocomial infections is a representative of genus Acinetobacter, A. baumannii. The mortality rate for nosocomial pneumonia reaches 35%. An important role in pathogenicity is played by factors that are active at all stages of the infectious process, determining tissue damage macroorganism and the survival of the microorganism. Despite the fact that A. baumannii has a higher pathogenicity for humans than other representatives of the genus, representatives of other species (A. calcoaceticus and A. junii) are also isolated from sputum samples of patients with community-acquired pneumonia (CAP). The aim of the study was to identify genes encoding pathogenicity factors of strains isolated from sputum samples of patients with СAP in medical organizations (MO) in Rostov-on-Don, as well as from swabs from objects of the external environment in the departments of medical organizations. The species identity of the strains was confirmed by the mass spectrometric method (MALDI-TOF). Genome-wide sequencing of genus Acinetobacter strains was performed on the Illumina MiSeq platform using the Nextera DNA Library Preparation Kit. The search for genes of pathogenicity factors of the studied strains was carried out using the Fragment Extractor program. The NCBI database was used as a database of nucleotide sequences. Among the isolated strains of genus Acinetobacter, 91.8% were A. baumannii, in solitary cases, A. calcoaceticus and A. junii were isolated.  It was determined that the studied strains possessed a wide range of genes for pathogenicity factors, which is similar both in clinical strains and in strains isolated from swabs from objects of the external environment in medical facilities, which apparently indicates their relationship.

Article Details

How to Cite
Gudueva Е. Н., Agafonova В. В. ., Gayevskaya Н. Е., Lychman В. А., Karnayxov А. Ю., & Chemisova О. С. . (2025). Molecular genetic characteristics of pathogenicity factors of genus Acinetobacter representatives isolated in medical organizations of Rostov-on-Don. Bulletin of Perm University. Biology, (2), 185–195. https://doi.org/10.17072/1994-9952-2025-2-185-195
Issue
No. 2 (2025): Bulletin of Perm University. BIOLOGY
Section
Микробиология

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Author Biographies

Victoria V. Agafonova, Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor, Rostov-on-Don, Russia

candidate of biology, Acting Head of the laboratory "Collection of pathogenic microorganisms"

Natalia E. Gayevskaya, Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor, Rostov-on-Don, Russia

candidate of medicine, Acting Director Institute

Valentina A. Lychman, Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor, Rostov-on-Don, Russia

laboratory assistant of the laboratory "Collection of pathogenic Microorganisms»

Alexandr Y. Karnayxov, Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor, Rostov-on-Don, Russia

Laboratory assistant of the laboratory "Collection of pathogenic Microorganisms»

Olga S. Chemisova, Don State Technical University, DSTU, Rostov-on-Don, Russia

candidate of biology, Associate Professor of the Department of Bioengineering
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