Benzoic acid-degrading halophilic strain Halomonas sp. D2

Article Sidebar

pdf (Русский)
Published: Dec 7, 2021
DOI: https://doi.org/10.17072/1994-9952-2021-3-171-177
Keywords:
halophilic bacteria Halomonas degradation of benzoic acid DNA sequencing benA gene

Main Article Content

Darya I. Usanina
Perm State University
https://orcid.org/0000-0003-0436-0890
Anna A. Pyankova
Institute of Ecology and Genetics of Microorganisms UB RAS
Elena G. Plotnikova
Institute of Ecology and Genetics of Microorganisms UB RAS
https://orcid.org/0000-0002-0107-0719

Abstract

Benzoic acid is widely used in various fields of industry, and it is a intermediate in the bacterial catabolism of many aromatic compounds, environmental pollutants. Halomonas sp. D2 strain, isolated from clay deposits of the salt mine of the Verkhnekamsky salt deposit (Solikamsk, Perm krai) is capable of using benzoic acid (BA) as a sole source of carbon and energy. Halomonas sp. D2 grows in the rich Raymond’s medium in the presence of 10-300 g/L NaCl and in the mineral medium with BA (1 g/L) in the presence of 30-70 g/L NaCl. The gene (benA) encoding the benzoate 1,2-dioxygenase alpha subunit was detected in the strain D2. Analysis of benA gene of the strain D2 showed the highest similarity (94.86%) with benA gene of Halomonas taeanensis BH539T with which the strain D2 is the most phylogenetically close in the 16S rRNA gene (99.43% similarity). The revealed properties of Halomonas sp. D2 strain makes it promising for use in biotechnologies for the restoration of saline soils and water contaminated with aromatic hydrocarbons.

Article Details

How to Cite
Usanina Д. И., Pyankova . А. А., & Plotnikova Е. Г. (2021). Benzoic acid-degrading halophilic strain Halomonas sp. D2. Bulletin of Perm University. Biology, (3), 171–177. https://doi.org/10.17072/1994-9952-2021-3-171-177
Issue
No. 3 (2021): Bulletin of Perm University. Biology
Section
Микробиология

License agreement

 

Author Biographies

Darya I. Usanina , Perm State University

Student

Anna A. Pyankova, Institute of Ecology and Genetics of Microorganisms UB RAS

Engineer of labo­ratory of microbiology of technogenic ecosystems

Elena G. Plotnikova, Institute of Ecology and Genetics of Microorganisms UB RAS

Doctor of biology, head of the laboratory of microbiology of technogenic ecosystems

References

Егорова Д.О. и др. Деструкция ароматических углеводородов штаммом Rhodococcus wratislaviensis KT112-7, выделенным из отходов соледобывающего предприятия // Прикладная биохимия и микробиология. 2013. Т. 49, № 3. С. 267–278.

Егорова Д.О., Пьянкова А.А. Скрининг гена альфа-субъединицы бензоат диоксигеназы в бактериальных ассоциациях, полученных в результате селекции на (хлор)ароматических соединениях // Вестник Пермского университета. Сер. Биология. 2019. Вып. 4. С. 464–470.

Пьянкова А.А. и др. Характеристика бактерий, выделенных из рудника Верхнекамского месторождения солей (Пермский край) // Вестник Пермского университета. Сер. Биология. 2020. Вып. 4. С. 312–320.

Шлегель Г. Общая микробиология: пер. с нем. М.: Мир, 1987. 567 с.

Baggi G. et al. Co-metabolizm of di-and trichlorben-zoates in a 2-chlorobenzoate-degrading bacterial culture: Effect of the position and number of halo-substituents // International Biodeterioration and Biodegradation. 2008. Vol. 62, № 1. P. 57−64.

Chen Y. et al. Revealing the saline adaptation strategies of the halophilic bacterium Halomonas bei-menensis through highthroughput omics and transposon mutagenesis approaches // Science Reports. 2017. Vol. 7. P. 183−195.

Fathepure B.Z. Recent studies in microbial degrada-tion of petroleum hydrocarbons in hypersaline environments // Frontiers in Microbiology. 2014. Vol. 5. P. 173.

Field J.A., Sierra-Alvarez R. Microbial transformation of chlorinated benzoates // Reviews in Environmental Science and BioTechnology. 2008. Vol. 7. P. 191–210.

García M.T. et al. Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds // International Journal of Systematic and Evolutionary of Microbiology. 2004. Vol. 54, № 5. P. 1723−1728.

Kim D. et al. Molecular cloning and functional characterization of the genes encoding benzoate and p-hydroxybenzoate degradation by the halophilic Chromohalobacter sp. strain HS-2 // FEMS Microbiology Letters. 2008. Vol. 280, № 2. P. 235−241.

Parales R.E., Resnick S.M. Aromatic ring hydroxylat-ing dioxygenases // Pseudomonas / Eds. J.L. Ra-mos, R.C. Levesque. Boston: Springer, 2006. P. 287–340.

Pieper D.H. Aerobic degradation of polychlorinated biphenyls. // Applied Microbiology Biotechnology. 2005. Vol. 67, № 2. P. 170–191.

Raymond R.L. Microbial oxidation of n-paraffinic hydrocarbons // Developments in Industrial Microbiology. 1961. Vol. 2, № 1. P. 23–32.