The effect of sodium salts of pyrrolo[3,4-c]-pyrazol-3-ones and 1-phenylpyrazole-3-carboxamides on the functional activity of in-nate immune cells in experimental models of pathological condi-tions in mice

Abstract

The primary function of innate immune effector cells is the elimination of exogenous and endogenous pathogens. However, in some cases, the absorptive and microbicidal activity of phagocytes can be impaired, leading to the development of pathological conditions. In this regard, the creation of immunotropic drugs with mild modulating activity in relation to the phagocytic function of leukocytes is relevant. In this regard, sodium salts of pyrrolo[3,4-c]-pyrazol-3-ones (1a-b) and 1-phenylpyrazole-3-carboxamide (2a) are promising; in previous studies, they showed a modulating effect on the functions of phagocytic cells. In this study, we investigated the effect of sodium salts of pyrrolo[3,4-c]-pyrazol-3-ones (1a-b) and 1-phenylpyrazole-3-carboxamide (2a) on changes in the absorptive activity of peritoneal leukocytes and their microbicidal potential under conditions of two- and six-hour immobilization stress and acute inflammation. The following results were obtained during the study: sodium salts of pyrrolo[3,4-c]-pyrazol-3-ones (1a-b) and 1-phenylpyrazole-3-carboxamide (2a) neutralized the inhibition of leukocyte scavenging activity caused by six-hour immobilization, as well as the inhibition of the production of active oxygen radicals caused by two- and six-hour immobilization stress. The studied compounds (1a-b, 2a), introduced against the background of zymosan, abolished the stimulating effect of the latter on the absorptive activity of leukocytes, but did not cause modulation of the production of active radicals by leukocytes in zymosan-stimulated cultures.