Analysis of the influence of biphenyl, chlorinated/chlorohydroxybiphenyls, and their biotransformation products on the immune response and morphofunctional state of the liver

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Published: Apr 2, 2025
DOI: https://doi.org/10.17072/1994-9952-2025-1-103-115
Keywords:
Rhodococcus biphenyl PCB humoral immunity cell-mediated immunity

Main Article Content

Sergey V. Gein
Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS, Perm, Russia
https://orcid.org/0000-0002-0799-3397
Darya O. Egorova
Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS, Perm, Russia
Nikolay A. Korolyov
Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS, Perm, Russia
Natalia P. Loginova
Wagner Perm State Medical University, Perm, Russia
Tatyana I. Gorbunova
I.Ya. Postovsky Institute of Organic Synthesis Ural Branch of RAS, Yekaterinburg, Russia
Elisaveta S. Nagovitsyna
Perm State University, Perm, Russia

Abstract

It has been established that biphenyl and PCB 12 (3,4-dichlorobiphenyl) suppressed humoral immunity by reducing the number of antibody-producing cells in the spleen. After microbial degradation of the investigated compounds by the Rhodococcus ruber 25 strain over 7 and 14 days, the metabolites of PCB 12 and biphenyl continued to exert an inhibitory effect on the number of antibody-producing cells. Mixture R, consisting of chlorinated and hydroxylated derivatives of biphenyl, did not influence the humoral response but stimulated the cell-mediated response; this effect was negated following microbial degradation. Histological studies indicated that biphenyl, PCB 12, and mixture R led to the development of chronic hepatitis in the liver, characterized by signs of fatty and focal hydropic (centrolobular) dystrophy of hepatocytes. Vascular responses were observed, including congestion with signs of hemolysis of erythrocytes. Perivascular lymphohistiocytic infiltration was noted. Under the influence of metabolites formed during the degradation of the investigated compounds by the R. ruber P25 strain over 7 to 14 days, dystrophic changes in hepatocytes persisted (without necro-inflammatory reaction), and signs of reparative regeneration developed.

Article Details

How to Cite
Gein С. В., Egorova Д. О., Korolyov Н. А., Loginova Н. П., Gorbunova Т. И., & Nagovitsyna Е. С. (2025). Analysis of the influence of biphenyl, chlorinated/chlorohydroxybiphenyls, and their biotransformation products on the immune response and morphofunctional state of the liver. Bulletin of Perm University. Biology, (1), 103–115. https://doi.org/10.17072/1994-9952-2025-1-103-115
Issue
No. 1 (2025): Bulletin of Perm University. BIOLOGY
Section
Иммунология

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Author Biographies

Sergey V. Gein, Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS, Perm, Russia

Doctor of Medical Sciences, Professor

Darya O. Egorova, Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS, Perm, Russia

Doctor of Biological Sciences, Associate Professor, Senior Researcher

Nikolay A. Korolyov, Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS, Perm, Russia

Postgraduate Student

Natalia P. Loginova, Wagner Perm State Medical University, Perm, Russia

Doctor of Medical Sciences

Tatyana I. Gorbunova, I.Ya. Postovsky Institute of Organic Synthesis Ural Branch of RAS, Yekaterinburg, Russia

Doctor of Chemical Sciences

Elisaveta S. Nagovitsyna, Perm State University, Perm, Russia

Master's student
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