Resistance of Rhodococcus collection strains to the effects of ecotoxicant – ethoxylated nonylphenol

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Published: Dec 15, 2023
DOI: https://doi.org/10.17072/1994-9952-2023-4-337-348
Keywords:
bioremediation Rhodococcus ethoxylated nonylphenol resistance minimum inhibitory concentration biofilms CLSM

Main Article Content

Elena A. Bayandina
Perm State University, Perm, Russia
https://orcid.org/0000-0002-9266-3877
Anastasia V. Pozdeeva
Perm State University, Perm, Russia
Maria S. Kuyukina
Perm State University, Perm, Russia, Institute of Ecology and Genetics of Microorganisms Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Perm, Russia
https://orcid.org/0000-0002-9165-8363
Irina B. Ivshina
Perm State University, Perm, Russia, Institute of Ecology and Genetics of Microorganisms Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Perm, Russia
https://orcid.org/0000-0003-2558-4789

Abstract

Ethoxylated nonylphenol (ENP), a widely used surfactant, is one of the most common xenoestrogens, hormone–like xenobiotics, the accumulation of which in the environment has a negative impact on the endocrine system of animals and humans, thereby increasing the level of environmental risk. Natural processes of ENP biodegradation are hindered due to its high toxicity to soil and aquatic microorganisms, which requires the selection of resistant strains-biodegraders of this ecopolutant. The screening of 50 strains of actinobacteria of the genus Rhodococcus from the Regional Specialised Collection of Alkanotrophic Microorganisms (acronym IEGM, http://www.iegmcol.ru) on resistance to ENP and the effect of xenobiotics on the dynamics of the formation of rhodococcal biofilms was studied. The strains R. ruber IEGM 615, 1263, and R. rhodochrous IEGM 655, isolated from oil-polluted ecosystems, resistant to high (MIC> 125 g/l) concentrations of ENP, were selected. It has been shown that long-term (up to 72 h) cultivation of R. ruber IEGM 71 biofilms in the presence of 15 g/l ENP contributed to an increase in the adhesive activity of cells and the synthesis of an exopolymer matrix, which plays a major role in protecting bacteria from the toxic effects of ENP. The obtained data reveal the potential of rhodococci for detoxification of xenoestrogens from the group of alkylated phenols, what can be used in the development of biotechnological methods of environmental purification from these ecotoxicants.

Article Details

How to Cite
Bayandina Е. А., Pozdeeva А. В., Kuyukina М. С., & Ivshina И. Б. (2023). Resistance of Rhodococcus collection strains to the effects of ecotoxicant – ethoxylated nonylphenol. Bulletin of Perm University. Biology, (4), 337–348. https://doi.org/10.17072/1994-9952-2023-4-337-348
Issue
No. 4 (2023): Bulletin of Perm University. BIOLOGY
Section
Микробиология

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Author Biographies

Elena A. Bayandina, Perm State University, Perm, Russia

Student at the Department of Microbiology and Immunology, Perm State University

Anastasia V. Pozdeeva, Perm State University, Perm, Russia

Student at the Department of Microbiology and Immunology, Perm State University

Maria S. Kuyukina, Perm State University, Perm, Russia, Institute of Ecology and Genetics of Microorganisms Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Perm, Russia

Dr. Sc. (Biol.), Professor at the Department of Microbiology and Immunology, Perm State University; Leading Research Officer, Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences;

Irina B. Ivshina, Perm State University, Perm, Russia, Institute of Ecology and Genetics of Microorganisms Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Perm, Russia

Dr. Sc. (Biol.), Professor, Academician of RAS, Professor at the Department of Microbiology and Immunology, Perm State University; Head of the Laboratory, Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences;

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