Molecular genetic analysis for Fusarium species diversity in the winter triticale (×Triticosecale Wittm. ex A. Camus)

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Published: Mar 29, 2023
DOI: https://doi.org/10.17072/1994-9952-2023-1-65-75
Keywords:
Fusarium fusarium infection diagnostics Real-Time PCR reagents kit winter triticale species diagnostics sensitivity

Main Article Content

Alexey A. Shvartsev
LLC Syntol, Moscow, Russia
https://orcid.org/0000-0002-2786-9860
Maria L. Koroleva
LLC Syntol, Moscow, Russia
https://orcid.org/0000-0001-8746-6249
Sofia A. Blinova
LLC Syntol, Moscow, Russia
https://orcid.org/0000-0001-6782-8353
Valentina S. Rubets
RSAU – MTAA named after K.A. Timiryazev, Moscow, Russia
https://orcid.org/0000-0003-1233-8837
Vladimir V. Pylnev
RSAU – MTAA named after K.A. Timiryazev, Moscow, Russia
https://orcid.org/0000-0003-0400-0609

Abstract

Fusarium is a large genus of fungi and is of interest primarily because numerous species are important plant pathogens. Fusarium can infect about 200 species of cultivated crops and lead to significant yield losses. In this regard, it is necessary to diagnose plants to detect fungi of the Fusarium genus and determine the phytopathogenic load in crops in order to identify a latent infection. The aim of this study is to test developed primers on fungi DNA collection and winter triticale. In this research, the developed primers were tested on 24 samples of fungi, obtained from different collections and on 50 samples of winter triticale from RSAU-MTAA named after K.A. Timiryazev. Primers and probes were selected and tested to determine belonging to the genus Fusarium by real-time polymerase chain reaction (qPCR). There were no false-positive results in the analysis of other types of fungi and related microorganisms. Analytical sensitivity of the developed oligonucleotides isn’t less than 15 copies of plasmid with the target DNA region per qPCR reaction. Diagnostic kit was tested using various Real-Time PCR systems. The triticale samples were tested for the presence of F. avenaceum, F. poae, F. culmorum, F. oxysporum, F. sambucinum. The greatest defeat by Fusarium was in varieties: PRAG522, PRAG507, PRAG 519, OGM-1. The results of the study could be used for screening diagnostic tests.

Article Details

How to Cite
Shvartsev А. А. ., Koroleva М. Л. ., Blinova С. А. ., Rubets В. С. ., & Pylnev В. В. . (2023). Molecular genetic analysis for Fusarium species diversity in the winter triticale (×Triticosecale Wittm. ex A. Camus). Bulletin of Perm University. Biology, (1), 65–75. https://doi.org/10.17072/1994-9952-2023-1-65-75
Issue
No. 1 (2023): Bulletin of Perm University. BIOLOGY
Section
Mycology

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Author Biographies

Alexey A. Shvartsev , LLC Syntol, Moscow, Russia

Postgraduate student

Maria L. Koroleva, LLC Syntol, Moscow, Russia

Researcher

Sofia A. Blinova, LLC Syntol, Moscow, Russia

Researcher

Valentina S. Rubets, RSAU – MTAA named after K.A. Timiryazev, Moscow, Russia

PhD, Dr. biol. sci., professor

Vladimir V. Pylnev, RSAU – MTAA named after K.A. Timiryazev, Moscow, Russia

Dr. biol. sci., professor

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