Reproductive potential of male rats in the experimental model of wildfire

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Published: Nov 8, 2021
DOI: https://doi.org/10.17072/1994-9952-2021-1-70-76
Keywords:
spermatogenesis DNA methylation DNA fragmentation experimental model of wildfire rats

Main Article Content

Vera Vokina
FSBSI East-Siberian Institute of Medical and Ecological Research
Ekaterina Kapustina
FSBSI East-Siberian Institute of Medical and Ecological Research
https://orcid.org/0000-0002-2803-4048
Mikhail Novikov
FSBSI East-Siberian Institute of Medical and Ecological Research
https://orcid.org/0000-0002-6100-6292
Elizaveta Andreeva
FSBSI East-Siberian Institute of Medical and Ecological Research
https://orcid.org/0000-0002-3709-8676

Abstract

The assessment of the indicators of the reproductive potential of male white rats exposed to smoke for 1 month was carried out. Examination of exposed animals included determination of the spermatogenesis index and the level of DNA fragmentation and methylation in the testes and blood. A statistically significant increase in the level of genome-wide methylation in the blood of rats exposed to smoke was revealed. A morphometric study of the testis tissue revealed a violation of spermatogenesis indices in exposed rats.

Article Details

How to Cite
Vokina В. А., Kapustina Е. А., Novikov М. А., & Andreeva Е. С. (2021). Reproductive potential of male rats in the experimental model of wildfire. Bulletin of Perm University. Biology, (1), 70–76. https://doi.org/10.17072/1994-9952-2021-1-70-76
Issue
No. 1 (2021): Bulletin of Perm University. Biology
Section
Ecology

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Author Biographies

Vera Vokina , FSBSI East-Siberian Institute of Medical and Ecological Research

Candidate of biology, Research Scientist of the laboratory for biomodelling and translational medicine

Ekaterina Kapustina , FSBSI East-Siberian Institute of Medical and Ecological Research

Candidate of Medicine, Research Scientist of the laboratory for biomodelling and translational medicine

Mikhail Novikov , FSBSI East-Siberian Institute of Medical and Ecological Research

Candidate of biology, Senior Researcher of the laboratory for biomodelling and translational medicine

Elizaveta Andreeva , FSBSI East-Siberian Institute of Medical and Ecological Research

Junior Research Scientist of the laboratory for biomodelling and translational medicine

References

Вокина В.А. и др. Исследование воздействия эмис-сии от лесных пожаров на морфофункциональное состояние центральной нервной системы белых крыс // Гигиена и санитария. 2019. № 98(11). С. 1245–1250.

Дурнев А.Д и др. Оценка генотоксических свойств методом ДНК-комет in vitro МР 4.2.0014-10: метод. рекомендации. М., 2010. 15 с.

Звягинцев А.М. и др. Загрязнение воздуха на европейской части России и Украине в условиях жаркого лета 2010 г. // Известия РАН. Физика атмосферы и океана. 2011. № 47(6). С. 757–766.

Панов А.В. и др. Комплексный подход в оценке эмиссии углеродсодержащих газов от лесных пожаров в Сибири // Метеорология и гидроло-гия. 2018. № 5. С. 30–39.

Ухов Ю. И., Астраханцев А. Ф. Морфометрические методы в оценке функционального состояния семенников // Архив анатомии, гистологии и эмбриологии. 1983. № 84(3). С. 66–72.

Air quality monitoring in Moscow, 2010. Newsletter WHO collaborating centre for quality management and air pollution control at the Federal Environment Agency. Germany, 2010. № 46. P. 9–14.

Ambatipudi S. et al. Tobacco smoking-associated genome-wide DNA methylation changes in the EPIC study // Epigenomics. 2016. Vol. 8, № 5. P. 599–618.

Bosse Y. et al. Molecular signature of smoking in human lung tissues // Cancer research. 2012. Vol. 72. P. 3753–3763.

Chen Y. et al. The impact of the fine ambient particle on infertile male's sperm quality // Urological Science. 2019. Vol. 30, № 4. P. 177–183.

Deng Z. et al. Association between air pollution and sperm quality: A systematic review and meta-analysis // Environ. Pollut. 2016. Vol. 208 (Pt B). P. 663–669.

Ewa B., Danuta M.S. Polycyclic aromatic hydrocar-bons and PAH-related DNA adducts // Journal of applied genetics. 2017. Vol. 58, № 3. P. 321–330.

Hansen C. et al. The effect of ambient air pollution on sperm quality // Environ Health Perspect. 2010. Vol 118, № 2. P. 203–209.

Huang X. et al. Association of exposure to ambient fine particulate matter constituents with semen quality among men attending a fertility center in China // Environ Sci. Technol. 2019. Vol. 53, № 10. P. 5957–5965.

Jenkins T.G. et al. Cigarette smoking significantly al-ters sperm DNA methylation patterns // Andrology. 2017. Vol. 5, № 6. P. 1089–1099.

Kiziler A.R. et al. High levels of cadmium and lead in seminal fluid and blood of smoking men are asso-ciated with high oxidative stress and damage in infertile subjects // Biological trace element research. 2007. Vol. 120. P. 82–91.

Kohli A. et al. Secondhand smoke in combination with ambient air pollution exposure is associated with increasedx CpG methylation and decreased expression of IFN-gamma in T effector cells and Foxp3 in T regulatory cells in children // Clinical epigenetics. 2012. Vol. 4. P. 17.

Kopp B., Zalko D., Audebert M. Genotoxicity of 11 heavy metals detected as food contaminants in two human cell lines // Environmental and molecular mutagenesis. 2018. Vol. 59, № 3. P. 202–210.

Kulikauskas V., Blaustein D., Ablin R. J. Cigarette smoking and its possible effects on sperm // Fertili-ty and sterility. 1985. Vol. 44. P. 526–534.

Liu X. et al. Lead induces genotoxicity via oxidative stress and promoter methylation of DNA repair genes in human lymphoblastoid TK6 cells // Medi-cal science monitor. 2018. Vol. 22, № 24. P. 4295–4304.

Miller S.R., Cherrington N.J. Transepithelial transport across the blood-testis barrier // Reproduction. 2018. Vol. 56, № 6. P. 187–194.

Murphy P.J. et al. Paternal cigarette smoke alters DNA methylation in sperm and gene expression in offspring brain // BioRxiv. 2019. 750638. doi: https://doi.org/10.1101/750638

Muthusamy S., Peng C., Ng J.C. Genotoxicity evaluation of multi-component mixtures of polyaromatic hydrocarbons (PAHs), arsenic, cadmium, and lead using flow cytometry based micronucleus test in HepG2 cells // Mutation research. Genetic toxicol-ogy and environmental mutagenesis. 2018. Vol. 827. P. 9–18.

Radwan M. et al. Exposure to ambient air pollutiondoes it affect semen quality and the level of repro-ductive hormones? // Ann. Hum. Biol. 2016. Vol. 43. P. 50–56.

Rolland M. et al. Decline in semen concentration and morphology in a sample of 26 609 men close to general population between 1989 and 2005 in France // Hum. Reprod. 2013. Vol. 28, № 2. P. 462–470.

Tsaprouni L.G. et al. Cigarette smoking reduces DNA methylation levels at multiple genomic loci but the effect is partially reversible upon cessation // Epigenetics. 2014. Vol. 9. P. 1382–1396.

Verma V. et al. Physicochemical and toxicological profiles of particulate matter in Los Angeles during the October 2007 southern California wildfires // Environ Sci. Technol. 2009. Vol. 43. P. 954–960.

Wentzel J.F. et al. Assessing the DNA methylation sta-tus of single cells with the comet assay // Analyti-cal Biochemistry. 2010. Vol. 400, № 2. P. 190–194.

Zeilinger S. et al. Tobacco smoking leads to extensive genome-wide changes in DNA methylation // PLoS One. 2013. Vol. 8, e63812.