MICROBIAL COMMUNITY OF THE TECHNOGENICALLY POLLUTED SOIL OF SALT-MINING REGION (BEREZNIKI)

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Published: Dec 16, 2018
Keywords:
denaturing gradient gel electrophoresis 16S rRNA gene nonculturable bacteria ortho-phthalate biphenyl Halomonas

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Ольга/Olga Викторовна/Victorovna Ястребова/Yastrebova
Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences
Анна/Anna Олеговна/Olegovna Воронина/Voronina
Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences
Людмила/Ludmila Николаевна/Nikolaevna Ананьина/Anan’ina
Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences
Екатерина/Ekaterina Сергеевна/Sergeyevna Корсакова/Korsakova
Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences; Perm State University
Елена/Elena Генриховна/Genrikhovna Плотникова/Plotnikova
Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences; Perm State University

Abstract

With the use of molecular-genetic methods a study of the structure of microbial community from the soil located near the BKPRU-3 salt separator of PAO Uralkali (Berezniki, Perm Territory) enterprise was carried out. The presence of 16S rRNA bacterial genes in the amount of 6,6 х 1011 (±8,87 х 107) copies of the gene per gram of soil and the Gram-negative bacteria dominance were detected by the real-time-PCR of the 16S rRNA gene fragments in the total DNA of the soil sample. The DGGE-analysis of 16S rDNA genes, amplified from total DNA, revealed that the bacterial communities of enrichment cultures, obtained on biphenyl and ortAo-phthalate, differed significantly from each other, as well as from the original community of the soil sample, both in composition and in the presence of dominant species. It was established that gram-negative bacteria of Gammaproteobacteria and Acidobacteria classes dominated in the derived enrichment cultures. By the method of DGGE-analysis "uncultivated" bacteria of the genus Halomonas were detected in the investigated microbial community.

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How to Cite
Ястребова/Yastrebova О. В., Воронина/Voronina А. О., Ананьина/Anan’ina Л. Н., Корсакова/Korsakova Е. С., & Плотникова/Plotnikova Е. Г. (2018). MICROBIAL COMMUNITY OF THE TECHNOGENICALLY POLLUTED SOIL OF SALT-MINING REGION (BEREZNIKI). Bulletin of Perm University. Biology, (2), 188–194. Retrieved from https://press.psu.ru/index.php/bio/article/view/1999
Issue
No. 2 (2018): Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology
Section
Микробиология

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Author Biographies

Ольга/Olga Викторовна/Victorovna Ястребова/Yastrebova, Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences

Candidate of biology, researcher of laboratory of molecular microbiology and biotechnology

Анна/Anna Олеговна/Olegovna Воронина/Voronina, Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences

Engineer of laboratory of molecular microbiology and biotechnology

Людмила/Ludmila Николаевна/Nikolaevna Ананьина/Anan’ina, Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences

Candidate of biology, researcher of laboratory of molecular microbiology and biotechnology

Екатерина/Ekaterina Сергеевна/Sergeyevna Корсакова/Korsakova, Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences; Perm State University

Candidate of biology, researcher of laboratory of molecular microbiology and biotechnology;Associate professor of the Department of botany and plant genetics

Елена/Elena Генриховна/Genrikhovna Плотникова/Plotnikova, Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences; Perm State University

Doctor of biology, leading researcher of laboratory of molecular microbiology and biotechnology;Professor of the Department of botany and plant genetics

References

Бачурин Б.А. Эколого-геохимическая характеристика отходов калийного производства // Горный журнал. 2008. Т. 10. С. 88–91.

Бачурин Б.А., Одинцова Т.А. Отходы горно-обогатительного производства как источники эмиссии органических поллютантов // Горный информационно-аналитический бюллетень. 2009. Вып. 7. С. 374–380.

Бачурин Б.А., Одинцова Т.А., Первова Е.С. Эколого-геохимическая характеристика флотореагентов // Материалы II Всерос. науч. виртуал. онлайн-конф. «Химическая наука: современные достижения и историческая перспектива». Казань, 2014. С. 17–22.

Воронина А.О. и др. Гены деструкции ароматических соединений в микробном сообществе почвы, загрязнённой отходами калийного производства (г. Березники, Пермский край) // Материалы X Всерос. конгресса молодых учёных-биологов «Симбиоз-Россия-2017». Казань, 2017. С. 73–74.

Кандаурова Ю.М., Ястребова О.В., Плотникова Е.Г. Бактерии-деструкторы орто-фталевой кислоты, выделенные из района добычи и переработки калийных солей (г. Березники, Пермский край) // Материалы XIV Всерос. науч.-практ. конф. с междунар. участием «Биодиагностика состояния природных и природно-техногенных систем». Киров, 2016. С. 329–332.

Корсакова Е.С., Пьянкова А.А., Плотникова Е.Г. Бактерии-деструкторы стойких органических загрязнителей – эфиров фталевой кислоты, как основа для создания новых экобиотехнологий // Известия Самарского научного центра РАН, 2013. Т. 15, № 3(5). С. 1633–1636.

Корсакова Е.С. и др. Разнообразие бактерий-деструкторов органических загрязнителей шламохранилища калийного производства (г. Березники, Пермский край) // Российский иммунологический журнал. 2015. Т. 9, № 2. С. 591–593.

Назаров А.В. и др. Влияние нефтяного загрязнения на бактерии дерново-подзолистой почвы // Почвоведение. 2010. Т. 12. С. 1489–1493.

Панов А.В. и др. Влияние загрязнения почвы на состав микробного сообщества // Микробиология. 2013. Т. 82, № 2. С. 239–246.

Розанова Е.П., Назина Т.Н. Углеводородокисляющие бактерии и их активность в нефтяных пластах // Микробиология. 1982. Т. 51. С. 324–348.

Castle D.M., Montgomery M.T., Kirchman D.L. Effects of naphthalene on microbial community composition in the Delaware estuary // FEMS Microbiol. Ecol. 2006. Vol. 56. P. 55–63.

Dickinson I. et al. Extremophiles in an Antarctic marine ecosystem // Microorganisms. 2016. Vol. 4(1). Р. 8. doi:10.3390/microorganisms4010008.

Feris K.P. et al. A shallow BTEX and MTBE contaminated aquifer supports a diverse microbial community // FEMS Microb. Ecol. 2004. Vol. 48. P. 589–600.

Fierer N. et al. Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays // Applied And Environmental Microbiology. 2005. Vol. 71, № 7. P. 4117–4120.

Gutierrez T. et al. Role of bacterial exopolysaccharides (EPS) in the fate of the oil released during the deepwater horizon oil spill // PLOS ONE. June 2013. Vol. 8, № 6. e67717.

Muyzer G., de Waal E.C., Uitterlinden A.G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA // Applied and Environmental Microbiology. 1993. Vol. 59. P. 695–700.

Puspita I.D. et al. Are uncultivated bacteria really uncultivable? // Microbes and Environments. 2012. Vol. 27, № 4. Р. 356–366.

Tiirola M.A. et al. Isolation and characterization of Novosphingobium sp. strain MT1, a dominant polychlorophenol-dagrading strain in a groundwater bioremediation system // Applied and Environmental Microbiology. 2002. Vol. 68. P. 173–180.

Vin˜as M. et al. Bacterial community dynamics and polycyclic aromatic hydrocarbon degradation during bioremediation of heavily creosote-contaminated soil // Applied and Environmental Microbiology. 2005. Vol. 71, № 11. P. 7008–7018.

Zaitsev G.M. et al. Genetic control of degradation of chlorinated benzoic acids in Arthrobacter globiformis, Corynebacterium sepedonicum and Pseudomonas cepacia strains // FEMS Microbiol. Lett. 1991. Vol. 81. P. 171–176.

References

Bachurin B.A. [Ecological and geochemical characteristics of the potash production wastes]. Gornyj žurnal. V. 10 (2008): pp. 88-91. (In Russ.).

Bachurin B.A., Odintsova T.A. [Wastes from mining and processing industry as sources of emission of organic pollutants]. Gornyj informacionnoanalitičeskij bjulleten’. Iss. 7 (2009): pp. 374-380. (In Russ.).

Bachurin B.A., Odintsova T.A., Pervova E.S. [Ecological and geochemical characteristics of flotation agents]. Materialy II Vserossijskoj naučnoj virtual’noj onlajn-konferencii “Chimičeskaja nauka: sovremennye dostiženija i istoričeskaja ptrspektiva” [Materials of the II-nd All-Russian scientific virtual online conference «Chemistry: advancements and historical perspective»]. Kazan, 2014, pp. 17-22. (In Russ.).

Voronina A.O., Korsakova E.S., Anan’ina L.N., Plotnikova E.G. [Genes of destruction of aromatic compounds in the microbial community of soil polluted with waste from potash production (Berezniki, Perm Region)]. Materialy X Vserossijskogo kongressa molodych učenych biologov “Simbioz-Rossija-2917” [Materials of X All-Russian Congress of Young Scientists-Biologists «Symbiosis-Russia-2017»]. Kazan, 2017, pp. 73-74. (In Russ.).

Kandaurova Yu.M., Yastrebova O.V., Plotnikova E.G. [The bacteria-destructors of ortho-phthalic acid, isolated from the area of extraction and processing of potassium salts. Berezniki, Perm region]. Materialy XIV Vserossijskoj naučnopraktičeskoj konferencii “Biodiagnostoka sostojanija prirodnych i prirodno-technogennych system” [Materials of XIV All-Russian scientificpractical conference with international participation «Biodiagnostics of natural and man-madetechnogenic systems»]. Kirov, 2016, pp. 329-332. (In Russ.).

Korsakova E.S., Pyankova A.A., Plotnikova E.G. [Bacteria-destructors of persistent organic pollutants – phthalic acid esters, as a basis for the creation of new ecobiotechnology]. Izvestija Samarskogo naučnogo centra RAN. V. 15, N 3(5) (2013): pp. 1633-1636. (In Russ.).

Korsakova E.S., Shestakova E.A., Khayrulina E.A., Nazarov A.V. [Variety of bacteria-destructors of organic pollutants of the slurry storage of potash production (Berezniki, Perm Territory)]. Rossijskij immunologičeskij žurnal. V. 9, N 2 (2015): pp. 591-593. (In Russ.).

Nazarov A.V., Ananyina L.N., Yastrebova O.V., Plotnikova E.G. [Influence of oil pollution on bacteria of sod-podzolic soil]. Počvovedenie. V. 12 (2010): pp. 1489-1493. (In Russ.).

Panov A.V., Esikova T.Z., Sokolov S.L., Kosheleva I.A., Boronin A.M. [Influence of soil pollution on the composition of the microbial community]. Mikrobiologija. V. 82, N 2 (2013): pp. 239-246. (In Russ.).

Rozanova E.P., Nazina T.N., [Hydrocarbon-Oxidizimg Bacteria and Their Activity in Oil-Bearing Beds]. Mikrobiologija. V. 51 (1982): pp. 324-348. (In Russ.).

Castle D.M., Montgomery M.T., Kirchman D.L. Effects of naphthalene on microbial community composition in the Delaware estuary. FEMS Microbiol. Ecol. V. 56 (2006): pp. 55-63.

Dickinson I., Goodall-Copestake W., Thorne M., Schlitt T., Ávila-Jiménez M., Pearce D. Extremophiles in an Antarctic marine ecosystem. Microorganisms. V. 4(1) (2016): p. 8.

doi:10.3390/microorganisms4010008

Feris K.P., Hristova K., Gebreyesus B., Mackay D., Scow K.M. A shallow BTEX and MTBE contaminated aquifer supports a diverse microbial community. FEMS Microb. Ecol. V. 48 (2004): pp. 589-600.

Fierer N., Jackson J.A., Vilgalys R., Jackson R.B. Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays. Applied and Environmental Microbiology. V. 71, N 7 (2005): pp. 4117-4120.

Gutierrez T., Berry D., Yang T., Mishamandani S., McKay L., Teske A., Aitken M.D. Role of bacterial exopolysaccharides (EPS) in the fate of the oil released during the deepwater horizon oil spill. PLOS ONE. V. 8, N 6 (June 2013) e67717.

Muyzer G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology. V. 59 (1993): pp. 695-700.

Tiirola M.A., Männistö M.K., Puhakka J.A., Kulomaa M.S. Isolation and characterization of Novosphingobium sp. Strain MT1, a dominant polychlorophenol-dagrading strain in a groundwater bioremediation system. Applied and Environmental Microbiology. V. 68 (2002): pp. 173-180.

Vin˜as M., Sabate J., Espuny M.J., Solanas A.M. Bacterial community dynamics and polycyclic aromatic hydrocarbon degradation during bioremediation of heavily creosote-contaminated soil. Applied and Environmental Microbiology. V. 71, N 11 (2005): pp. 7008-7018.

Zaitsev G.M., Tsoi T.V., Grishenkov V.G., Plotnikova E.G., Boronin A.M. Genetic control of degradation of chlorinated benzoic acids in Arthrobacter globiformis, Corynebacterium sepedonicum and Pseudomonas cepacia strains. FEMS Microbiol. Lett. V. 81 (1991): pp. 171-176.

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