MICROClONAL PROPAGATION CITRUS LIMON (L.) OSBECK CULTIVAR PAVLOVSKY

Abstract

There is the results of clonal micropropagation of Citrus limon L. Osbeck of Pavlovsky variety. It was established that the following procedure of the use of sterilizing agents is most suitable for the sterilization of explants: 96% ethanol (5 sec) and 8% sodium hypochlorite solution (20 min). The yield of the sterile culture was 70%. It was found that the solid MS nutrient medium with the addition of 1.0 mg/l benzylaminopurine and 0.5 mg/l naphthylacetic acid was optimal to introduce the lemon Pavlovsky into the culture in vitro. The yield of viable explants was 77.5%. Cultivation of microshoots was carried out on DKW medium with the addition of phytohormones 2.0 mg/l benzylaminopurine and 2.0 mg/l gibellic acid. 2.45±0.78 child microshoots was get from each parental explant. Optimum for rhizogenesis of regenerants is medium ½MS with addition of 1.0 mg/l naphthylacetic acid and 1.0 mg/l indolylacetic acid. The percentage of rooted microshoots was 65.1. Plants obtained from in vitro culture were transferred to conditions of closed soil in the soil substrate: peat, perlite, vermiculite, sand in a ratio of 7:2:3:2. Plant survival was 87.5%.