THE EVALUATION OF THE ENDOGENOUS MELATONIN IMPACT ON REGULATORY T CELLS FUNCTIONAL ACTIVITY DURING PREGNANCY

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Published: Nov 3, 2018
Keywords:
Treg differentiation pregnancy melatonin melatonin receptors

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Наталья/Natalya Сергеевна/Sergeevna Глебездина/Glebezdina
Institute of Ecology and Genetics of Microorganism UB RAS
Анна/Anna Александровна/Alexandrovna Олина/Olina
Perm State Medical University named after academician E.A Wagner
Ирина/Irina Валерьевна/Valeryevna Некрасова/Nekrasova
Institute of Ecology and Genetics of Microorganism UB RAS
Елена/Elena Михайловна/Michajlovna Куклина/Kuklina
Institute of Ecology and Genetics of Microorganism UB RAS

Abstract

Regulatory T lymphocytes (Treg) play an important role in the physiological course of pregnancy providing the formation of peripheral tolerance. The paper presents data of the ability of the epiphyseal hormone melatonin to regulate the differentiation of this T cell subpopulation during gestation. The subjects of the study were leukocytes of women in I and Ш trimester of pregnancy as well as leukocytes of healthy non-pregnant women of reproductive age. The level and activity of Treg were evaluated ex vivo and in vitro in response to polyclonal activation (anti-CD3/CD28) by expression of the differentiation marker of the Treg - transcription factor FoxP3 by flow cytometry and by the production of the key cytokine of these cells - TGFp by ELISA. The contribution of endogenous melatonin in the regulation of Treg was determined by cultivation of lymphocytes in the presence of autologous serum against the background of blockade of melatonin-dependent signals using the inhibitor of membrane melatonin receptors (MT1 / MT2). It was shown that the differentiation process was differently regulated by endogenous melatonin in pregnant and non-pregnant women. An increase of the percentage of CD4+FoxP3+ T cells was observed only in non-pregnant cells under the polyclonal activation (anti-CD3/CD28), and this increase did not cancel by the blockade of melatonin-dependent signals. In pregnant women Treg differentiation depended on MT1/MT2-mediated signals.

Article Details

How to Cite
Глебездина/Glebezdina Н. С., Олина/Olina А. А., Некрасова/Nekrasova И. В., & Куклина/Kuklina Е. М. (2018). THE EVALUATION OF THE ENDOGENOUS MELATONIN IMPACT ON REGULATORY T CELLS FUNCTIONAL ACTIVITY DURING PREGNANCY. Bulletin of Perm University. Biology, (4), 457–462. Retrieved from https://press.psu.ru/index.php/bio/article/view/1893
Issue
No. 4 (2017): Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology
Section
Клиническая лабораторная диагностика

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Author Biographies

Наталья/Natalya Сергеевна/Sergeevna Глебездина/Glebezdina, Institute of Ecology and Genetics of Microorganism UB RAS

Candidate of biology, junior researcher of laboratory immunoregulation

Анна/Anna Александровна/Alexandrovna Олина/Olina, Perm State Medical University named after academician E.A Wagner

Doctor of medical Sciences, professor of the Department of Obstetrics and Gynecology

Ирина/Irina Валерьевна/Valeryevna Некрасова/Nekrasova, Institute of Ecology and Genetics of Microorganism UB RAS

Candidate of biology, researcher of laboratory immunoregulation

Елена/Elena Михайловна/Michajlovna Куклина/Kuklina, Institute of Ecology and Genetics of Microorganism UB RAS

Doctor of biology, leading researcher of laboratory immunoregulation

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