FACTORS PERSISTENCE OF STAPHYLOCOCCI ISOLATED FROM BACTERmCARRIERS

Article Sidebar

PDF (Русский)
Published: Oct 29, 2018
Keywords:
staphylococcus stability to lysozyme biofilm formation bacteriocarrier

Main Article Content

Екатерина/Ekaterina Михайловна/M. Гордина/Gordina
St. Petersburg State Pediatric Medical University
Эдуард/Edward Семенович/S. Горовиц/Horowitz
Perm Medical State University named by acad. E.A. Vagner
Светлана/Svetlana Валерьевна/V. Поспелова/Pospelova
Perm Medical State University named by acad. E.A. Vagner

Abstract

92 healthy subjects were examined on staphylococcal bacteriocarrier. 113 strains of staphylococci, including S. aureus - 27, coagulase-negative staphylococci (CNS) - 86 were isolated from the mucous of the nose and throat. Resistance to lysozyme and biofilm formation ability isolates of staphylococcus, and the relationship of the studied factors were estimated. Cultures S. aureus differed more pronounced resistance to the action of lysozyme compared with CNS (the differences are statistically significant). About half of all tested cultures were characterized by a high degree of expression of biofilm formation. At the same strains with weak activity biofilm formation mainly occurred among CNS. An analysis of the interdependence of degree of resistance to lysozyme and the intensity of biofilm formation showed that all studied strains of staphylococci, the degree of manifestation of resistance to the action of lysozyme and the intensity of the biofilm formation are inversely related (r = -0,35). In other words, in strains with a high degree of resistance to lysozyme usually recorded low degree of biofilm formation. It is found that in the presence of lysozyme biofilms formed by different staphylococcal species, are less pronounced in comparison with the control. The studies found that all studied strains of staphylococci are characterized by resistance to lysozyme and the ability to form a biofilm of varying severity. Also found an inverse correlation between the studied factors of persistence. We also show the value of lysozyme, as a factor in reducing biofilm formation of staphylococcus.

Article Details

How to Cite
Гордина/Gordina Е. М., Горовиц/Horowitz Э. С., & Поспелова/Pospelova С. В. (2018). FACTORS PERSISTENCE OF STAPHYLOCOCCI ISOLATED FROM BACTERmCARRIERS. Bulletin of Perm University. Biology, (2), 205–209. Retrieved from https://press.psu.ru/index.php/bio/article/view/1855
Issue
No. 2 (2017): Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology
Section
Клиническая лабораторная диагностика

License agreement

 

Author Biographies

Екатерина/Ekaterina Михайловна/M. Гордина/Gordina, St. Petersburg State Pediatric Medical University

MD, PhD, Senior Lecturer Department of Microbiology, Virology and Immunology

Эдуард/Edward Семенович/S. Горовиц/Horowitz, Perm Medical State University named by acad. E.A. Vagner

PhD in medicine, professor, head department of microbiology, virology with the course of clinical laboratory diagnostic

Светлана/Svetlana Валерьевна/V. Поспелова/Pospelova, Perm Medical State University named by acad. E.A. Vagner

PhD in medicine, assistant professor of microbiology, virology with the course of clinical laboratory diagnostic

References

Бухарин О.В. Микросимбиоценоз. Екатеринбург, 2014. 260 с.

Гостев В.В., Сидоренко С.В. Бактериальные биопленки и инфекции // Журнал инфектологии. 2010. Т. 2, № 3. С. 4-15.

Ильина Т.С., Романова Ю.М., Гинцбург А.Л. Биопленки как способ существования бактерий в окружающей среде и организме хозяина: феномен, генетический контроль и системы регуляции их развития // Генетика. 2004. Т. 40, № 11. С. 1445-1456.

Поспелова С.В., Горовиц Э.С., Лемкина Л.М. Влияние техногенной нагрузки на видовой состав и особенности биопленкообразования стафилококков, изолированных от бактерионосителей // Экология человека. 2014. № 5. С. 4852.

Чеботарь И.В. Механизмы антибиопленочного иммунитета // Вестник РАМН. 2012. № 12. С. 22-29.

Archer N.K. et al. Staphylococcus aureus biofilms: properties, regulation and roles in human disease // Virulence. 2011. Vol. 2, № 5. P. 445-459.

Gotz F. Staphylococcus and biofilms // Mol. Microb. 2002. Vol. 43, № 6. P. 1367-1378

Kaplan J.B. Biofilm dispersal: mechanisms, clinical implications, and potential therapeutic uses // J. Dent. Res. 2010. Vol. 89, № 3. Р. 205-218.

Stepanovic S. et al. Quantification of biofilm in mi-crotiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci // APMIS. 2007. Vol. 115, № 8. P. 891-899.

Yuan S., Wan D., Liang B. Lysozyme-coupled poly(poly(ethylene glycol) methacrylate)-stainless steel hybrids and their antifouling and antibacterial surfaces // Langmuir. 2011. Vol. 27, № 6. P. 2761-2774.

References

Buharin O.V. Mikrobosimbiocenoz [Microsymbio-cenosis]. Ekaterinburg, 2014, 260 p. (In Russ.).

Gostev V.V., Sidorenko S.V. [Bacterial biofilms and infections]. Zurnal infektologii, V. 2, N 3 (2010): pp. 4-15. (In Russ.).

Il'ina T.S., Romanova Ju.M., Gincburg A.L. [Biofilms as a way of existence of bacteria in the environment and the host organism: the phenomenon, genetic control and the systems of regulation of their development]. Genetika, V. 40, N 11 (2004): pp. 1445-1456. (In Russ.).

Pospelova S.V., Horowitz E.S., Lemkina L.M. Influence of anthropogenic load on species composition and peculiarities of biofilm formation of staphylo-cocci isolated from bacterial carriers. Ëkologija celoveka, N 5 (2014): pp. 48-52. (In Russ.).

Chebotar' I.V. [Mechanisms of antibiofilm immunity]. Vestnik RAMN, N 12 (2012): pp. 22-29. (In Russ.).

Archer N.K., Mazaitis M.J., Costerton J.W., Leid J.G., Powers М.Е., Shirtliff М.Е. Staphylococcus aureus biofilms: properties, regulation and roles in human disease. Virulence, V. 2, N 5 (2011): pp. 445-459.

Gotz F. Staphylococcus and biofilms. Mol. Microb. V. 43, N 6 (2002): pp. 1367-1378.

Kaplan J.B. Biofilm dispersal: mechanisms, clinical implications, and potential therapeutic uses. J. Dent. Res. V. 89, N 3 (2010): pp. 205-218.

Stepanovic S., Vukovic D., Hola V., Di Bonaventura G., Djukic S., Cirkovic I. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS. V. 115, N 8 (2007): pp. 891-899.

Yuan, S. Wan D., Liang B. Lysozyme-coupled poly(poly(ethylene glycol) methacrylate)-stainless steel hybrids and their antifouling and antibacterial surfaces. Langmuir, V. 27, N 6 (2011): pp. 2761-2774.