STRUCTURAL AND FUNCTIONAL CHARACTERISTICS OF BACTERIAL CONSORTIUM, ISOLATED FROM TECHNOGENIC SOILS OF THE VERKHNEKAMSK SALT DEPOSIT

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Published: Oct 25, 2018
Keywords:
biodegradation polycyclic aromatic hydrocarbons halotolerant and halophilic bacteria bacterial communities

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Ольга/Olga Викторовна/Viktorovna Ястребова/Yastrebova
Institute of Ecology and Genetics of Microorganism UB RAS
Ирина/Irina Адольфовна/Adolfovna Кошелева/Kosheleva
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms
Елена/Elena Генриховна/Genrikhovna Плотникова/Plotnicova
Institute of Ecology and Genetics of Microorganism UB RAS; Perm State University

Abstract

The consortium of microorganisms SMB1, capable of efficient growing on naphthalene as a sole source of carbon and energy in the presence of 10% NaCl has been isolated from soil of potassium salt mining region (Berezniki, Perm krai). The consortium consists of two halotolerant bacterial strains-destructors of naphthalene, namely Arthrobacter sp. (strains SMB145 =ВКМ Ac-2551, SMB11 =ВКМ Ac-2552), halotolerant strain Brevibacterium permense SMB14 (=ВКМ Ас-2280Т =LMG 22207Т) and halophile strain Chromohalobacter sp. SMB17 incapable of naphthalene utilization. During co-cultivation of the three strains Arthrobacter sp. SMB145, Brevibacterium permense SMB14 and Chromohalobacter sp. SMB17 on naphthalene at 7% NaCl higher growth parameters of the strain destructor Arthrobacter sp. SMB145 have been observed compared to individual strain cultivation. The bacterial consortium SMB1 respiration rate was higher in comparison to the individual strain SMB145 cultivation on naphthalene at 5% NaCl. Isolated bacterial consortium SMB1 can be recommended for the development of new biotechnologies for the treatment of polluted soils and wastewater under the conditions of salinization.

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How to Cite
Ястребова/Yastrebova О. В., Кошелева/Kosheleva И. А., & Плотникова/Plotnicova Е. Г. (2018). STRUCTURAL AND FUNCTIONAL CHARACTERISTICS OF BACTERIAL CONSORTIUM, ISOLATED FROM TECHNOGENIC SOILS OF THE VERKHNEKAMSK SALT DEPOSIT. Bulletin of Perm University. Biology, (3), 253–262. Retrieved from https://press.psu.ru/index.php/bio/article/view/1817
Issue
No. 3 (2016): Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology
Section
Микробиология

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Author Biographies

Ольга/Olga Викторовна/Viktorovna Ястребова/Yastrebova, Institute of Ecology and Genetics of Microorganism UB RAS

Researcher of laboratory of molecular microbiology and biotechnology

Ирина/Irina Адольфовна/Adolfovna Кошелева/Kosheleva, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms

Senior researcher of laboratory of plasmids biology

Елена/Elena Генриховна/Genrikhovna Плотникова/Plotnicova, Institute of Ecology and Genetics of Microorganism UB RAS; Perm State University

Leading researcher of laboratory of molecular microbiology and biotechnology;Professor of the Department of botany and plant genetics

References

Ананьина Л.Н., Алтынцева О.В., Плотникова Е.Г. Изучение сообщества микроорганизмов, выделенного из района солеразработок // Вестник Пермского университета. 2005. Вып. 6. Биология. С. 109-114.

Ананьина Л.П. и др. Salinicola socius gen. nov., sp. nov. - новая умеренно галофильная бактерия из ассоциации микроорганизмов, утилизирующей нафталин // Микробиология. 2007. Т. 76, № 3. С. 369-376.

Гавриш Е.Ю. и др. Три новых вида бревибактерий - Brevibacterium antiquum sp. nov., Brevibacte-rium aurantiacum sp. nov. и Brevibacterium per-mense sp. nov. // Микробиология. 2004. T. 73, № 2. С. 218-225.

Кашнер Д. Жизнь микробов в экстремальных условиях. М.: Мир. 1981. 365 с.

Определитель бактерий Берджи: пер. с англ. / под ред. Дж. Хоулта и др. М.: Мир. 1997. Т. 1, 2.

Плотникова Е.Г. и др. Бактерии-деструкторы полициклических ароматических углеводородов, выделенные из почв и донных отложений района солеразработок // Микробиология. 2001. Т. 70, № 1. С. 61-69.

Плотникова Е.Г. и др. Галотолерантные бактерии рода Arthrobacter - деструкторы полициклических ароматических углеводородов // Экология. 2011. №6. С. 459-466.

Розанова Е.П., Назина Т.Н. Углеводородокисляю-щие бактерии и их активность в нефтяных пластах//Микробиология. 1982. Т. 51. С. 324-348.

Ястребова О.В., Ананьина Л.Н., Плотникова Е.Г. Бактерии рода Bacillus, выделенные из почв района солеразработок // Вестник Пермского университета. 2008. Вып. 9 (25). Биология. С. 58-62.

Anan'ina L.N. et al. Naphthalene-degrading bacteria of the genus Rhodococcus from the Verkhne-kamsk salt mining region of Russia // Antonie Van Leeuwenhoek. 2011. Vol. 100, Is. 2. P. 309-316.

Al-Mailem D.M., Eliyas M., Radwan S.S. Oil-bioremediation potential of two hydrocarbonoclas-tic, diazotrophic Marinobacter strains from hy-persaline areas along the Arabian Gulfcoasts // Extremophiles. 2013. Vol. 17. P. 463-470.

Arahal D.R. et al. Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov. // Int J Syst Evol Microbiol. 2001. Vol. 51. P. 1443-1448.

Ashok, T., Saxena, S., Musarrat J. Isolation and characterization of four polycyclic aromatic hydrocarbon degrading bacteria from soil near an oil refinery // Lett. Appl. Microbiol. 1995. Vol. 21, Is. 4. P. 246-248.

Berlendis S. et al. First evidence of aerobic biodégradation of BTEX compounds by pure cultures of Marinobacter II Appl. Biochem. Biotechnol. 2010. Vol. 160. P. 1992-1999.

Bonfa M.R.L. et al. Phenol degradation by halophilic bacteria isolated from hypersaline environments // Biodégradation. 2013. Vol. 24. P. 699-709.

Bouchez M., Blanchetl D., Vandecasteele J.-P. An interfacial uptake mechanism for the degradation of pyrene by a Rhodococcus strain // Microbiology. 1997. Vol. 143. P. 1087-1093.

Casellas M. et al. Isolation and characterization of a fluorine-degrading bacterial strain and its role in synergistic degradation of fluorene by a consortium // Can. J. Microbiol. 1998. Vol. 44. P. 734-742.

Castle D.M., Montgomery M.T., Kirchman D.L. Effects of naphthalene on microbial community composition in The Delaware estuary // FEMS Microbiol. Ecol. 2006. Vol. 56. P. 55-63.

Cerniglia C.E. Biodégradation of polycyclic aromatic hydrocarbons // Biodégradation. 1992. Vol. 3. P. 351-368.

Dalvi S., Azetsu S., Patrauchan M.A., Aktas D.F., Fa-thepure B.Z. Proteogenomic elucidation of the initial steps in the benzene degradation pathway of a novel halophile, Arhodomonas sp. Strain Rozel, isolated from a hypersaline environment // Appl. Environ. Microbiol. 2012. Vol. 78. P. 7309-7316.

Dastgheib S.M.M. et al. Biodégradation of polycyclic aromatic hydrocarbons by a halophilic microbial consortium // Appl. Microbiol. Biotechnol. 2012. Vol. 95. P. 789-798.

Ensign J.C., Rittenberg S.C. A crystalline pigment produced from 2-hydro-xypridine by Arthrobacter crvstallopoites n.sp. // Arch.Mikrobiol. 1963. Vol. 47. P. 137-153.

Feng T.-C. et al. Phenanthrene biodégradation by halophilic Martelella sp. AD-3 // Journal of Applied Microbiology. 2012. Vol. 113. P. 779-789.

Ferrero M. et al.. Coexistence of two distinct copies of naphthalene degradation genes in Pseudomonas strains isolated from the western mediterranean region // Appl. Environ. Microbiol. 2002. Vol. 68. P. 957-962.

Guo G. et al. Effect of salt contents on enzymatic activities and halophilic microbial community structure during phenanthrene degradation // International Biodeterioration & Biodégradation. 2016. Vol. 110. P. 8-15.

Habe H, Omori T. Genetics of Polycyclic Aromatic Hydrocarbon Metabolism in Diverse Aerobic Bacteria // Biosci. Biotechnol. Biochem. 2003. Vol. 67, №2. P. 225-243.

Kim, D. et al. Molecular cloning and fimctional characterization of the genes encoding benzoate and p-hydroxybenzoate degradation by the halophilic Chro-mohalobacter sp. strain HS-2 // FEMS Microbiol. Lett. 2008. Vol. 280. P. 235-241.

Kumar M. et al. A halotolerant and thermotolerant Bacillus sp. degrades hydrocarbons and produces tensionactive emulsifyingagent // World J. Microbiol. Biotechnol. 2007. Vol. 23. P. 211-220.

Martins L.F., Peixoto R.S. Biodégradation of petroleum hydrocarbons in hypersaline environments // Brazilian Journal of Microbiology. 2012. P. 865-872.

McGenity T.J. Halophilic hydrocarbon degraders. In: K. N. Timmis (ed). Handbook of Hydrocarbon and Lipid Microbiology. Berlin: Springer-Verlag, 2010. P. 1939-1951.

Moghadam M.S. et al. Biodégradation of polycyclic aromatic hydrocarbons by a bacterial consortium enriched from mangrove sediments // Journal of Environmental Health Science & Engineering. 2014. 12:114 URL: http://www.ijehse.eom/content/12/l/114.

Pec,onek J. et al. Reclassification of Pseudomonas beijerinckii Hof 1935 as Chromohalobacter bei-jerinckii comb, nov., and emended description of the species // Int. J. Syst. Evol. Microbiol. 2006. Vol. 56. P. 1953-1957.

Sánchez-Porro C. et al. Chromohalobacter japonicus sp. nov., a moderately halophilic bacterium isolated from a Japanese salty food // Int. J. Syst. Evol. Microbiol. 2007. Vol. 57. P. 2262-2266.

Tagger S., Truffaut N., Le Petit J. Preliminary study on relationship among strains forming a bacterial community selected on naphthalene from a marine sediment // Can. J. Microbiol. 1990. Vol. 36. P. 676-681.

Tarn N.F. et al. Preliminary study on biodégradation of phenanthrene by bacteria isolated from mangrove sediments in Hong Kong. // Mar. Pollut. Bull. 2002. Vol. 45. P. 316-324.

Ventosa A., Nieto J. J., Oren A. Biology of moderately halophilic aerobic bacteria // Microbiology and Molecular biology reviews. 1998. Vol. 62, № 2. P. 504-544.

Versalovic J., Schneider M., de Bruijn F.J. Genomic fingerprinting of bacteria using repetitive sequence based polymerase chain reaction // Meth. Mol. Cell. Biol. 1994. Vol. 5. P. 25-40.

Zaidi B.R., Imam S.H. Factors afecting microbial degradation of Polycyclic Aromatic Hydrocarbon phenanthrene in the Caribbean Coastal Water // Marine Pollution Bulletin. 1999. Vol. 38, № 8. P. 737-742.

References

Anan'ina, L.N., Altyntseva O.V., Plotnikova, E.G., [The study of microbial community isolated from the region of salt mining], Vestnik Permskogo universiteta. 2005, Iss. 6. Biologija, pp. 109-114. (In Russ.).

Anan'ina, L.N., Plotnikova, E.G., Gavrish, E.Yu., et al. [Salinicola socius gen. nov., sp. nov., a Moderately Halophilic Bacterium from the Association of Naphthalene Degrading Microorganisms], Mikrobiologija, 2007, V. 76, N. 3, pp. 369-376. (In Russ.).

Gavrish, E.Yu., Krauzova, V.I., Potekhina, N.V., Karasev, S.G., Plotnikova, E.G., Altyntseva O.V., Korosteleva, L.A., Evtushenko, L.I., [Three New Species of Brevibacteria, Brevibacte-rium antiquum sp. nov., Brevibacterium auran-tiacum sp. nov., and Brevibacterium permense sp. nov.]. Microbiologija. 2004, V. 73, N. 2, pp. 218-225. (In Russ.).

Kushner, D.J. Zizn' mikrobov v ekstremal'nych us-lovijach [Microbial Life in Extreme Environments]. Moscow, Mir Publ., 1981. 365 p. (In Russ.).

Opredelitel' bakterij Berdzi [Bergey's Manual of Systematic Bacteriology Bergey]. Moscow, Mir Publ., 1997. V. 1, 2. (In Russ.).

Plotnikova E.G., Altyntseva O.V., Kosheleva I.A., Puntus I.F., Filonov A.E., Gavrish E.U., Dema-kov V.A., Boronin A.M. [Bacteria Decomposing Polycyclic Aromatic Hydrocarbons Isolated from Soil and Bottom Sediments in the Region of Salt Mines], Mikrobiologija, 2001, V. 70, N 1, pp. 61-69. (In Russ.).

Plotnikova E.G., Yastrebova O.V., Anan'ina L.N., Dorofeeva L.V., Lysanskaya V.Ya., Demakov V.A [Halotolerant bacteria of the genus Arthro-bacter degrading polycyclic aromatic hydrocarbons], Ecologija. 2011, V. 42, N 6, pp. 502-509. (In Russ.).

Rozanova E.P., Nazina T.N., [Hydrocarbon-Oxidizimg Bacteria and Their Activity in Oil-Bearing Beds]. Mikrobiologija, 1982, V. 51, pp. 324-348. (In Russ.).

Yastrebova O.V., Ananyina L.N, Plotnikova E.G. [Bacteria of the genus Bacillus isolated from soils in the area of salt mining], Vestnik Permskogo universiteta. 2008, Iss. 9. Biology, pp. 58-62. (In Russ.).

Anan'ina L.N., Yastrebova O.V., Demakov V.A., Plotnikova E.G. Naphthalene-degrading bacteria of the genus Rhodococcus from the Verkhne-kamsk salt mining region of Russia. Antonie Van Leeuwenhoek. 2011, V. 100,1. 2, pp. 309-316.

Al-Mailem D.M., Eliyas M., Radwan S.S. Oil-bioremediation potential of two hydrocarbono-clastic, diazotrophic Marinobacter strains from hypersaline areas along the Arabian Gulfcoasts. Extremophiles. 2013, V. 17, pp. 463-470.

Arahal D.R, Garcia M.T., Ludwig W., Schleifer K.H., Ventosa A. Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov. Int. J. Syst. Evol. Microbiol. 2001, V. 51, pp. 1443-1448.

Ashok, T., Saxena, S., Musarrat J. Isolation and characterization of four polycyclic aromatic hydrocarbon degrading bacteria from soil near an oil refinery. Lett. Appl. Microbiol. 1995, V. 21,1. 4, pp. 246-248.

Berlendis S., Cayol, J.-L., Verhe, F., Laveau S., Tholozan, J.-C., Ollivier, B. First evidence of aerobic biodégradation of BTEX compounds by pure cultures of Marinobacter. Appl. Biochem. Biotechnol. 2010, V. 160, pp. 1992-1999.

Bonfa M.R.L., Grossman M.J., Piubeli F., Mellado E., Durrant, L.R. Phenol degradation by halophilic bacteria isolated from hypersaline environments. Biodégradation. 2013, V. 24, pp. 699-709.

Bouchez M., Blanchetl D., Vandecasteele J.-P. An interfacial uptake mechanism for the degradation of pyrene by a Rhodococcus strain. Microbiology. 1997, V. 143, pp. 1087-1093.

Casellas M., Grifoll M., Sebate J., Solanas A.M. Isolation and characterization of a fluorine-degrading bacterial strain and its role in synergistic degradation of fluorene by a consortium. Can. J. Microbiol. 1998, V. 44, pp. 734-742.

Castle D.M., Montgomery M.T., Kirchman D.L. Effects of naphthalene on microbial community composition in The Delaware estuary. FEMS Microbiol. Ecol. 2006, V. 56, pp. 55-63.

Cerniglia C.E. Biodégradation of polycyclic aromatic hydrocarbons. Biodégradation, 1992, V. 3, pp. 351-368.

Dalvi S., Azetsu S., Patrauchan M.A., Aktas D.F., Fathepure B.Z. Proteogenomic elucidation of the initial steps in the benzene degradation pathway of a novel halophile, Arhodomonas sp. Strain Rozel, isolated from a hypersaline environment. Appl. Environ. Microbiol. 2012, V. 78, pp. 7309-7316.

Dastgheib S.M.M., Amoozegar M.A., Khajeh K., Shavandi M., Ventosa A. Biodégradation of polycyclic aromatic hydrocarbons by a halophilic microbial consortium. Appl. Microbiol. Biotechnol. 2012, V. 95, pp. 789-798.

Ensign J.C., Rittenberg S.C. A crystalline pigment produced from 2-hydro-xypridine by Arthrobacter crvstallopoites n. sp. Arch. Mikrobiol. 1963, V. 47, pp. 137-153.

Feng T.-C. Cui C.-Z., Dong F., Feng Y.-Y., Liu Y.-D. Yang X.-M. Phenanthrene biodégradation by halophilic Martelella sp. AD-3. Journal of Applied Microbiology. 2012, V. 113, pp. 779-789.

Ferrero M., Llobet Brossa E., Lalucat J. Garcia-Valdés E., Rossellô-Mora R., Bosch R. Coexistence of two distinct copies of naphthalene degradation genes in Pseudomonas strains isolated from the western mediterranean region. Appl. Environ. Microbiol. 2002, V. 68, pp. 957-962.

Guo G., He F., Tian F., Huang Y., Wang H. Effect of salt contents on enzymatic activities and halophilic microbial community structure during phenanthrene degradation. International Biodeterio-ration & Biodégradation. 2016, V. 110, pp. 8-15.

Habe H., Omori T. Genetics of Polycyclic Aromatic Hydrocarbon Metabolism in Diverse Aerobic Bacteria. Biosci. Biotechnol. Biochem. 2003, V. 67, N2, pp. 225-243.

Kim, D., Kim, S.W., Choi, K.Y., Lee, J.S. Kim, E. Molecular cloning and functional characterization of the genes encoding benzoate and p-hydroxybenzoate degradation by the halophilic Chromohalobacter sp. strain HS-2. FEMS Microbiol. Lett. 2008, V. 280, pp. 235-241.

Kumar M., Leon V., deSistro Materano A., Ilzins O.A. A halotolerant and thermotolerant Bacillus sp. degrades hydrocarbons and produces ten-sionactive emulsifyingagent. World J. Microbiol. Biotechnol. 2007, V. 23, pp. 211-220.

Martins L.F., Peixoto R.S. Biodégradation of petroleum hydrocarbons in hypersaline environments. Brazilian Journal of Microbiology. 2012, ISSN 1517-8382, pp. 865-872.

McGenity T.J. Halophilic hydrocarbon degraders. In: K. N. Timmis (ed). Handbook of Hydrocarbon and Lipid Microbiology. Berlin, Springer-Verlag, 2010. pp. 1939-1951.

Moghadam M. S., Ebrahimipour G., Abtahi B., Ghas-sempour A., Hashtroudi M.S. Biodégradation of polycyclic aromatic hydrocarbons by a bacterial consortium enriched from mangrove sediments. Journal of Environmental Health Science & Engineering. 2014. 12:114 http://www.ijehse.eom/content/12/l/l 14.

Pec,onek J., Gruber C., Gallego V., Ventosa A., Busse H.-J., Ka'mpfer P., Radax C., Stan-Lotter H. Reclassification of Pseudomonas beijerinckii Hof 1935 as Chromohalobacter beijerinckii comb, nov., and emended description of the species. Int. J. Syst. Evol. Microbiol. 2006, V. 56, pp. 1953-1957.

Sânchez-Porro C, Tokunaga H, Tokunaga M, Ventosa A. Chromohalobacter japonicus sp. nov., a moderately halophilic bacterium isolated from a Japanese salty food. Int. J. Syst. Evol. Microbiol. 2007, V. 57, pp. 2262-2266.

Tagger S., Truffaut N., Le Petit J. Preliminary study on relationship among strains forming a bacterial community selected on naphthalene from a marine sediment. Can. J. Microbiol. 1990, V. 36, pp. 676-681.

Tam N.F., Guo C.L., Yau W.Y., Wong Y.S. Preliminary study on biodégradation of phenanthrene by bacteria isolated from mangrove sediments in Hong Kong.. Mar. Pollut. Bull. 2002, V. 45, pp. 316-324.

Ventosa A., Nieto J.J., Oren A. Biology of moderately halophilic aerobic bacteria. Microbiology and Molecular biology reviews. 1998, V. 62, № 2, pp. 504-544.

Versalovic J., Schneider M., de Bruijn F.J. Genomic fingerprinting of bacteria using repetitive sequence based polymerase chain reaction // Meth. Mol. Cell. Biol. 1994, V. 5, pp. 25-40.

Zaidi B.R., Imam S.H. Factors afecting microbial degradation of Polycyclic Aromatic Hydrocarbon phenanthrene in the Caribbean Coastal Water. Marine Pollution Bulletin. 1999, V. 38, N 8, pp. 737-742.