BIOFILM FORMATION OF ESCHERICHIA COLI UNDER THE REDOX-STATUS MODIFICATION

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Published: Oct 25, 2018
Keywords:
Escherichia coli biofilm formation capacity thiol redox-systems oxidative stress disulfide stress

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Елена/Elena Владимировна/Vladimirovna Лепехина/Lepekhina
Institute of Ecology and Genetics of Microorganism UB RAS; Perm National Research Polytechnic University
Галина/Galina Васильевна/Vasil'evna Смирнова/Smirnova
Institute of Ecology and Genetics of Microorganism UB RAS
Олег/Oleg Николаевич/Nikolaevich Октябрьский/Oktyabrsky
Institute of Ecology and Genetics of Microorganism UB RAS; Perm National Research Polytechnic University

Abstract

The ability of biofilm formation in Escherichia coli bearing mutants in the components of glutathione and thioredoxin redox systems, as well as in the regulator of the general stress response RpoS was studied at different growth temperatures. Statistically significant correlation between specific bacteria biofilm formation and expression of the antioxidant gene katG was revealed at 37°C. This indicates a possible contribution of the OxyR regulon activation in the formation of bacteria biofilms. Monitoring of the sodA gene expression in thiol redox system mutants at 37°C showed 1.15-1.8-fold increase in gshA, gor, trxA, gshAtrxA gortrxB mutants. A direct relationship between the biofilm formation and expression of the gene sodA under the same conditions was established. The presence of mutations in components of the thiol redox systems significantly changed the production of biofilms under temperature stresses, which is probably due to the interruptions in the redox regulation of intracellular signaling pathways. It was shown the RpoS participation in biofilm formation at the optimum growth temperature and RpoS-dependent nature of biofilm formation induction in 24°C and 44°C.

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How to Cite
Лепехина/Lepekhina Е. В., Смирнова/Smirnova Г. В., & Октябрьский/Oktyabrsky О. Н. (2018). BIOFILM FORMATION OF ESCHERICHIA COLI UNDER THE REDOX-STATUS MODIFICATION. Bulletin of Perm University. Biology, (4), 338–343. Retrieved from https://press.psu.ru/index.php/bio/article/view/1770
Issue
No. 4 (2016): Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology
Section
Микробиология

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Author Biographies

Елена/Elena Владимировна/Vladimirovna Лепехина/Lepekhina, Institute of Ecology and Genetics of Microorganism UB RAS; Perm National Research Polytechnic University

Candidate of biology, engineer of Laboratory of Physiology and Genetics of Microorganisms;Associate Professor of the Department of Chemistry and Biotechnology

Галина/Galina Васильевна/Vasil'evna Смирнова/Smirnova, Institute of Ecology and Genetics of Microorganism UB RAS

Doctor of biology, leading researcher of Laboratory of Physiology and Genetics of Microorganisms

Олег/Oleg Николаевич/Nikolaevich Октябрьский/Oktyabrsky, Institute of Ecology and Genetics of Microorganism UB RAS; Perm National Research Polytechnic University

Doctor of biology, professor, director of Laboratory of Physiology and Genetics of Microorganisms;Professor of the Department of Chemistry and Biotechnology

References

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