Phylogenetic diversity of nitrile-utilizing bacteria of soda sludge storage facility and some of their physiological and biochemical fea-tures

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Published: Oct 30, 2025
DOI: https://doi.org/10.17072/1994-9952-2025-3-279-288
Keywords:
nitrile hydrolysis nitrile-utilizing bacteria soda sludge storage biofilm alkaliphiles halophiles haloalkalitolerant bacteria nitrile hydratase amidase

Main Article Content

Galina A. Syrovatskaya
Institute of Ecology and Genetics of Microorganisms, Ural Branch RAS, Perm, Russia
Aleksandr Yu. Maksimov
Institute of Ecology and Genetics of Microorganisms, Ural Branch RAS, Perm, Russia
Yuliya G. Maksimova
Institute of Ecology and Genetics of Microorganisms, Ural Branch RAS, Perm, Russia

Abstract

The phylogenetic diversity of nitrile-utilizing bacteria from a soda sludge storage facility was studied. Haloalkalitolerant bacteria were isolated on acetamide and acetonitrile as the only carbon sources and identified by 16S rDNA sequencing. The isolates were found to belong to the phyla Actinomycetota (Arthrobacter, Microbacterium, Rhodococcus spp.) and Pseudomonadota (Acinetobacter, Ensifer, Pseudomonas, Rhizobium spp.). The isolates were found to have nitrile hydratase and amidase activity and contain genes for two types of amidases and Fe-containing nitrile hydratase. No nitrilase activity or nitrilase genes, Co-containing nitrile hydratases, or enantioselective amidases were found. Isolates are capable of forming biofilms, and biofilm biomass increases with decreasing growth temperature.

Article Details

How to Cite
Syrovatskaya Г. А., Maksimov А. Ю. ., & Maksimova Ю. Г. . (2025). Phylogenetic diversity of nitrile-utilizing bacteria of soda sludge storage facility and some of their physiological and biochemical fea-tures. Bulletin of Perm University. Biology, (3), 279–288. https://doi.org/10.17072/1994-9952-2025-3-279-288
Issue
No. 3 (2025): Bulletin of Perm University. BIOLOGY
Section
Микробиология

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Author Biographies

Galina A. Syrovatskaya, Institute of Ecology and Genetics of Microorganisms, Ural Branch RAS, Perm, Russia

postgraduate student

Aleksandr Yu. Maksimov, Institute of Ecology and Genetics of Microorganisms, Ural Branch RAS, Perm, Russia

Ph.D. in Biology, Associate Professor, Senior Research Fellow

Yuliya G. Maksimova, Institute of Ecology and Genetics of Microorganisms, Ural Branch RAS, Perm, Russia

doctor of biology, Associate Professor, head of the laboratory
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