Antioxidant and antibacterial potential of epiphytic microorganisms from terrestrial and aquatic plants
DOI:
https://doi.org/10.17072/1994-9952-2026-2-159-168Keywords:
epiphytic microorganisms, antioxidants, antibacterial properties, biologically active substancesAbstract
The paper examines in detail the ecological and physiological prerequisites for the formation of antioxidant and antibacterial potentials, including adaptation to extreme phyllosphere conditions (UV radiation, ambient pH, etc.), symbiotic interactions with host plants, and evolutionary mechanisms of protective metabolite synthesis. Key methodological approaches are summarized, ranging from from cultural isolation methods and molecular techniques (16S rRNA sequencing, metagenomics) to functional Screening using the DPPH (2,2-diphenyl-1-picrylhydrazyl) stable free radical scavenging assay for antioxidants and zones of inhibition for antimicrobial substances. In addition, promising areas for the biotechnological use of epiphytes are identified, including the development of probiotic preparations based on beneficial strains (Pseudomonas fluorescens, Bacillus subtilis) and the integration of epiphytes into agronomy to increase plant stress tolerance. This review study focuses on the systematization and comprehensive analysis of current scientific data on the metabolic potential of epiphytic microorganisms acting as producers of antioxidant and antimicrobial compounds. Most of the studies reviewed in this article focus on identifying and characterizing bioactive compounds produced by epiphytes, which primarily inhabit marine algae. The results of further research in this area may contribute to the development of new and promising preparations.References
Список источников
1. Галиева Г.Ш., Галицкая П.Ю., Селивановская С.Ю. Растительный микробиом: происхождение, состав и функции // Ученые записки Казанского университета. Сер. Естественные науки. 2023. Т. 165, кн. 2. С. 231–262. DOI: 10.26907/2542-064X.2023.2.231-262 EDN: TJOOQC
2. Козубенко А.А., Рудакова Л.В. Современные методы определения антиоксидантной активности // Молодежный инновационный вестник. 2018. Т. 7, № S1. C. 314. URL: https://new.vestnik-surgery.com/index.php/2415-7805/article/view/4224 (дата обращения: 30.03.2026). EDN: YXJXAN
3. Куросава Э. Вещество, вызывающее болезнь «дурных побегов» риса // Труды Сельскохозяй-ственного общества Токио. 1926. Т. 2. С. 411–424.
4. Мусин Р.Р. и др. Влияние комбинации гомоферментативных и гетероферментативных молочно-кислых бактерий на качество силоса люцерны // Вестник Алтайского государственного аграрного университета. 2022. № 1 (207). С. 89–94. DOI: 10.53083/1996–4277-2022-207-1-89-94 EDN: AXQQBN
5. Приходько С.И., Селицкая О.В. Исследование качественного состава эпифитных микроорганиз-мов некоторых культурных и сорных растений Тамбовской и Московской областей // Агрохимиче-ский вестник. 2014. № 6. С. 40–43. EDN: SGNLSD
6. Сиротин А.А. и др. Изучение эпифитной микрофлоры некоторых овощных культур // Междуна-родный журнал прикладных и фундаментальных исследований. 2016. № 7-2. С. 230–232. URL: https://applied-research.ru/ru/article/view?id=9799 (дата обращения: 07.04.2026). EDN: WAYERH
7. Ябута Т., Сумки Ю. On the crystal of gibberellin, a substance to promote plant growth // Journal of the Agricultural Chemical Society of Japan. 1938. Т. 14, № 12. С. 1526–1531.
8. Яшин А.Я. Методология определения антиоксидантной активности и содержания антиоксидан-тов в образцах растительного и животного происхождения // J. Analytics. 2018. URL: https://www.j-analytics.ru/ journal/article/9028 (дата обращения: 30.03.2026).
9. Adeolu F.J. et al. Comparative antioxidant potentials and high performance liquid chromatography of leaf extracts of Citrus sinensis and its epiphytes // Достижения современной науки. 2020. № 7. С. 95–96.
10. Backer R. et al. Plant growth promoting rhizobacteria: context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture // Frontiers in Plant Science. 2018. Vol. 9. Art. 1473. DOI: 10.3389/fpls.2018.01473 EDN: VMBDGU
11. Bartelme R.P. et al. Stripping away the soil: plant growth promoting microbiology opportunities in aquaponics // Frontiers in Microbiology. 2018. Vol. 9. Art. 8. DOI: 10.3389/fmicb.2018.00008
12. Gershman M.D. et al. Multistate outbreak of Pseudomonas fluorescens bloodstream infection after exposure to contaminated heparinized saline flush prepared by a compounding pharmacy // Clinical Infec-tious Diseases. 2008. Vol. 47, № 11. P. 1372–1379. DOI: 10.1086/592968
13. Glick B.R. Plant Growth Promoting Bacteria: Mechanisms and Applications // Scientifica. 2012. № 2012. Art. 963401. DOI: 10.6064/2012/963401
14. Hirano S.S., Upper C.D. Ecology and epidemiology of phyllosphere yeasts in some Wisconsin cran-berry marshes // Canadian Journal of Microbiology. 1991. Vol. 37. P. 194–201.
15. Horta A. et al. Antioxidant and antimicrobial potential of the Bifurcaria bifurcata epiphytic bacte-ria // Marine Drugs. 2014. Vol. 12, № 3. P. 1676–1687. DOI: 10.3390/md12031676 EDN: SOSXQF
16. Pawar V., Majeed A., Pal V., Singh A. Antioxidant activity of Sphaerococcus coronopifolius and its Epiphytic bacteria // Brazilian Journal of Microbiology. 2015. Vol. 46, № 2. P. 531–538. DOI: 10.1590/S1517-838246220140384
17. Penesyan A., Kjelleberg S., Egan S. Development of novel drugs from marine surface associated mi-croorganisms // Marine Drugs. 2011. Vol. 9, № 12. С. 2696–2717.
18. Phytochemical composition and in vitro antioxidant activities of Citrus sinensis peel extracts // J. Pharm. Bioallied Sci. 2018. Vol. 10, № 3. С. 133–139.
19. Romano G. et al. Secondary metabolites from marine bacteria and fungi as potential antibiotics // Marine Drugs. 2022. Vol. 20, № 5. Art. 317.
20. Schlechter R et al. Next generation husbandry: The microbiology of plant surfaces as a path to nov-el disease control / R. Schlechter et al. // Current Opinion in Plant Biology. 2019. Vol. 49. С. 65–71.
21. Sharma A. et al. Comparative analysis of antioxidant and antimicrobial potential of epiphytic and endophytic bacteria from medicinal plants // Journal of Applied Microbiology. 2023. Vol. 134, № 3. Art. 045. DOI: 10.1093/jambio/lxad045
References
1. Galieva G.Sh., Galitskaya P.Yu., Selivanovskaya S.Yu. [Plant microbiome: origin, composition and functions]. Uchenye zapiski Kazanskogo universiteta. Seriya: Estestvennye nauki .V. 165, iss. 2 (2023) pp. 231-262. (In Russ.) https://doi.org/10.26907/2542-064X.2023.2.231-262. EDN: TJOOQC
2. Kozubenko A.A., Rudakova L.V. [Modern methods for determining antioxidant activity]. Mo-lodežnyj innovacionnyj vestnik, V. 7, No. S1 (2018): pp. 42-48. (In Russ,). Available at: https://new.vestnik-surgery.com/index.php/2415-7805/article/view/4224 (accessed 30.03.2026).
3. Kurosawa E. [A substance causing the “foolish seedling” disease of rice]. Trudy Sel’skochozjajstvennogo obščestva Tokio, V. 2 (1926): pp. 411-424. (In Russ.).
4. Musin R.R., Tremasova A.M., Skvortsov E.V., Bykova P.V., Eroshin A.I., Akhtyamova A.M. [Effect of a combination of homofermentative and heterofermentative lactic acid bacteria on the quality of alfalfa silage]. Vestnik Altajskogo gosudarstvennogo agrarnogo universiteta. No. 1 (2022): pp. 89-94. (In Russ.). DOI: 10.53083/1996–4277-2022-207-1-89-94. EDN: AXQQBN
5. Prikhodko S.I., Selitskaya O.V. (2014). [Study of the qualitative composition of epiphytic microor-ganisms of some cultivated and weed plants in Tambov and Moscow regions]. Agrochimičeskij vestnik. No. 6 (2014): pp. 40-43. EDN: SGNLSD
6. Sirotin A.A., Zelenkova V.N., Shkuropat M.N., Kortyukova E.A. (2016). [Study of epiphytic micro-flora of some vegetable crops]. Meždunarodnyj žurnal prikladnych i fundamental’nych issledovanij. No. 7-2 (2016): pp. 230-232. (In Russ.). Available at: https://applied-research.ru/ru/article/view?id=9799 (ac-cessed 07.04.2026). EDN: WAYERH
7. Yabuta T., Sumiki Yu. On the crystal of gibberellin, a substance to promote plant growth. Journal of the Agricultural Chemical Society of Japan. V. 14, No. 12 (1938): pp. 1526-1531.
8. Yashin A.Ya. Methodology for determining antioxidant activity and content of antioxidants in plant and animal samples. J Analytics. (2018). Available at: https://www.j-analytics.ru/journal/article/9028 (ac-cessed 30.03.2026).
9. Adeolu F.J., Oluwaseun A.A., Olawale J.T., Racheal A.J., Adedoyin O.B., Mary A.A. et al. (2020). Comparative antioxidant potentials and high performance liquid chromatography of leaf extracts of Citrus sinensis and its epiphytes. Dostiženija sovremennoj nauki. No. 7 (2020): pp. 95-96.
10. Backer R., Rokem J.S., Ilangumaran G., Lamont J., Praslickova D., Ricci E., Subramanian S., Smith D.L. Plant growth promoting rhizobacteria: context, mechanisms of action, and roadmap to commercializa-tion of biostimulants for sustainable agriculture. Frontiers in Plant Science. V. 9 (2018). Art. 1473. DOI: 10.3389/fpls.2018.01473. EDN: VMBDGU
11. Bartelme R.P., Oyserman B.O., Blom J.E., Sepulveda Villet O.J., Newton R.J. Stripping away the soil: plant growth promoting microbiology opportunities in aquaponics. Frontiers in Microbiology, V. 9 (2018). Art. 8. DOI: 10.3389/fmicb.2018.00008.
12. Gershman M.D., Kennedy D.J., Noble Wang J., Kim C., Gullion J., Kacica M. et al. Multistate out-break of Pseudomonas fluorescens bloodstream infection after exposure to contaminated heparinized sa-line flush prepared by a compounding pharmacy. Clinical Infectious Diseases. V. 47, No. 11 (2008): pp. 1372-1379. DOI: 10.1086/592968
13. Glick B.R. Plant Growth Promoting Bacteria: Mechanisms and Applications. Scientifica, No. 2012 (2012). Art. 963401. DOI: 10.6064/2012/963401.
14. Hirano S.S., Upper C.D. Ecology and epidemiology of phyllosphere yeasts in some Wisconsin cran-berry marshes. Canadian Journal of Microbiology. V. 37 (1991): pp. 194-201.
15. Horta A., Pinteus S., Alves C., Fino N., Silva J., Fernandes S., Rodrigues A., Pedrosa R. Antioxidant and antimicrobial potential of the Bifurcaria bifurcata epiphytic bacteria. Marine Drugs. V. 12, No. 3 (2014): pp. 1676-1687. doi: 10.3390/md12031676. EDN: SOSXQF
16. Pawar V., Majeed A., Pal V., Singh A. Antioxidant activity of Sphaerococcus coronopifolius and its epiphytic bacteria. Brazilian Journal of Microbiology. V. 46, No. 2 (2015): pp. 531-538. DOI: 10.1590/S1517-838246220140384
17. Penesyan A., Kjelleberg S., Egan S. Development of novel drugs from marine surface associated mi-croorganisms. Marine Drugs. V. 9, No. 12 (2011): pp. 2696-2717.
18. Phytochemical composition and in vitro antioxidant activities of Citrus sinensis peel extracts. Journal of Pharmacy and Bioallied Sciences. V. 10, No. 3 (2018): pp. 133-139.
19. Romano G. et al. Secondary metabolites from marine bacteria and fungi as potential antibiotics. Marine Drugs. V. 20, No. 5 (2022). Art. 317.
20. Schlechter R. et al. Next generation husbandry: The microbiology of plant surfaces as a path to novel disease control. Current Opinion in Plant Biology. V. 49 (2019): pp. 65-71.
21. Sharma A., Puri S., Sehgal R., Kumar V., Sharma V. Comparative analysis of antioxidant and antimi-crobial potential of epiphytic and endophytic bacteria from medicinal plants. Journal of Applied Microbi-ology. V. 134, No. 3 (2023). Art. 045. DOI: 10.1093/jambio/lxad045.
