Biofilms of hydrocarbon-oxidizing bacteria from anthropogenic dis-turbed soils in Kogalym

Authors

  • Anastasia S. Korobeynikova Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia
  • Dmitrii M. Golubev Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia
  • Anna K. Tarasyuk Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia
  • Olga S. Glinskaya Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia
  • Denis V. Utkin Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia
  • Olga V. Nechaeva Saratov State Technical University named after Yuri Gagarin, Saratov, Russia
  • Elena V. Glinskaya Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

DOI:

https://doi.org/10.17072/1994-9952-2025-4-406-414

Keywords:

biofilms, hydrocarbon-oxidizing microorganisms, soil, oil pollution, bioremediation

Abstract

The article is devoted to the determination of the biological properties and conditions of biofilm formation by hydrocarbon-oxidizing bacteria for their further use in oil pollution treatment and remediation of anthropogenic disturbed soils. The object of the study is hydrocarbon-oxidizing microorganisms isolated from soil samples from Kogalym: Bacillus alcalophilus WS-3027B, B. funiculus LY-2403G, B. halodurans MH-3011N, B. niacini TC-8101S, B. psychrodurans LV-1106E, Curtobacterium flaccumfaciens AE-0851V. Biofilm formation activities were evaluated by the crystal violet staining method for assessing biofilm formation degree in the stationary phase according to O'Toole et al. Among the hydrocarbon-oxidizing microorganisms, B. alcalophilus WS-3027B and C. flaccumfaciens AE-0851V were found to form dense biofilms, while B. halodurans MH-3011N, B. niacini TC-8101S, B. funiculus LY-2403G, and B. psychrodurans LV-1106E exhibited moderate biofilm production. Optimal conditions for the formation of biofilms by the studied microorganisms have been established. The conducted study of biofilm formation of hydrocarbon-oxidizing bacteria opens up prospects for the use of these strains as active destructors of petroleum products in the bioremediation of contaminated soils.

Author Biographies

  • Anastasia S. Korobeynikova, Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

    Master's student of the Department of Microbiology and Plant Physiology, Faculty of Biology, N.G. Chernyshevsky SSU

  • Dmitrii M. Golubev, Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

    Master's student of the Department of Microbiology and Plant Physiology, Faculty of Biology, N.G. Chernyshevsky SSU

  • Anna K. Tarasyuk, Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

    Postgraduate student, Laboratory assistant at the Department of Microbiology and Plant Physiology, Faculty of Biology, N.G. Chernyshevsky SSU

  • Olga S. Glinskaya, Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

    Student, Department of Romance and Germanic Philology and Translation Studies, Institute of Philology and Journalism, N.G. Chernyshevsky SSU

  • Denis V. Utkin, Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

    Doctor of Biological Sciences, Professor, Head of the Department of Microbiology and Plant Physiology, Faculty of Biology, N.G. Chernyshevsky SSU

  • Olga V. Nechaeva, Saratov State Technical University named after Yuri Gagarin, Saratov, Russia

    Doctor of Biological Sciences, Associate Professor, Leading Researcher, Laboratory of Molecular Microbiology, Department of Molecular Microbiology and Bioinformatics, Institute of Microbiology, FSBI «National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov» Ministry of Health of the Russian Federation; Professor of the Department of Medical Microbiology named after Academician Z.V. Ermolyeva FSBEI FPE "Russian Medical Academy of Continuous Professional Education" of the Ministry of Healthcare of the Russian Federation

  • Elena V. Glinskaya, Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

    Candidate of Biological Sciences, Associate Professor, Department of Microbiology and Plant Physiology, Faculty of Biology, N.G. Chernyshevsky SSU

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Published

2025-12-23

Issue

No. 4 (2025): Bulletin of Perm University. BIOLOGY

Section

Микробиология

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How to Cite

Biofilms of hydrocarbon-oxidizing bacteria from anthropogenic dis-turbed soils in Kogalym. (2025). Bulletin of Perm University. Biology, 4, 406-414. https://doi.org/10.17072/1994-9952-2025-4-406-414

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