Влияние ионов меди и цинка на зеленую микроводоросль Chlorella vulgaris
DOI:
https://doi.org/10.17072/1994-9952-2026-2-139-149Ключевые слова:
Chlorella vulgaris, ионы меди и цинка, хлорофиллы, каротиноиды, углеводы, пролин, токсичностьАннотация
Представлены результаты исследования токсического действия меди и цинка в диапазоне концентраций 1–17 мкМ Cu2+ и 5–60 мкМ Zn2+ на пресноводную зеленую микроводоросль Chlorella vulgaris IMBR-19. В недельном эксперименте оценку токсичности тяжелых металлов давали по таким показателям, как численность живых и мертвых клеток, содержание внутриклеточных пигментов, углеводов и пролина. Установлено, что наибольшим угнетающим действием по отношению к хлорелле обладает медь. Пороговыми концентрациями, при которых рост культуры не наблюдается, а количество мертвых клеток приближается к 100%, были 13 и 40 мкМ для меди и цинка соответственно. Установлено, что при увеличении концентрации тяжелых металлов (в диапазоне роста микроводоросли) не происходит статистически достоверного снижения концентрации фотосинтетических пигментов. Для концентраций 7–11 мкМ меди обнаружен существенный рост содержания хлорофиллов и каротиноидов. Полученные данные свидетельствуют, что при повышении концентрации тяжелых металлов происходит увеличение содержания углеводов в клетках хлореллы в 2 и 3 раза по сравнению с контролем в случае меди и цинка соответственно. Аналогичная зависимость была выявлена и в случае с пролином, где опытные варианты с медью и цинком при 13 мкМ и 30 мкМ соответственно превышали контроль в 2.7 и 4 раза.Библиографические ссылки
Список источников
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