BIOFILM FORMATION OF ESCHERICHIA COLI UNDER THE REDOX-STATUS MODIFICATION
Keywords:
Escherichia coli, biofilm formation capacity, thiol redox-systems, oxidative stress, disulfide stressAbstract
The ability of biofilm formation in Escherichia coli bearing mutants in the components of glutathione and thioredoxin redox systems, as well as in the regulator of the general stress response RpoS was studied at different growth temperatures. Statistically significant correlation between specific bacteria biofilm formation and expression of the antioxidant gene katG was revealed at 37°C. This indicates a possible contribution of the OxyR regulon activation in the formation of bacteria biofilms. Monitoring of the sodA gene expression in thiol redox system mutants at 37°C showed 1.15-1.8-fold increase in gshA, gor, trxA, gshAtrxA gortrxB mutants. A direct relationship between the biofilm formation and expression of the gene sodA under the same conditions was established. The presence of mutations in components of the thiol redox systems significantly changed the production of biofilms under temperature stresses, which is probably due to the interruptions in the redox regulation of intracellular signaling pathways. It was shown the RpoS participation in biofilm formation at the optimum growth temperature and RpoS-dependent nature of biofilm formation induction in 24°C and 44°C.References
Николаев Ю.А., Плакунов В.К. Биопленка - «Город микробов» или аналог многоклеточного организма? // Микробиология. 2007. Т. 76. С.149-163.
Октябрьский О.Н., Смирнова Г.В. Редокс регуляция клеточных функций // Биохимия. 2007. Т. 72. С. 158-174.
Baba Т. et al. Construction of Escherichia coli strain К-12 in-frame, single-gene knockout mutants: the Keio Collection // Mol. Syst. Biol. 2006. Vol. 2. P. 1-11.
Be loin C., Roux A., Ghigo J-M. Escherichia coli biofilms // Curr. Top Microbiol. Immunol. 2008. Vol. 322. P. 249-289.
Mah Т.Е. et al. A genetic basis for Pseudomonas aeruginosa biofilm antibiotic resistance // Nature. 2003. V. 426. P. 306-310.
Malpica R. et al. Identification of a quinone-sensitive redox switch in the ArcB sensor kinase // Proc. Natl. Acad. Sei. 2004. Vol. 101. P. 13318-13323.
Mika F., Hengge R. Small Regulatory RNAs in the control of motility and biofilm Formation in E. coli and Salmonella II Int. J. Mol. Sei. 2013. Vol. 14. P. 4560-4579.
Miller J. N. Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory Press, 1972.
Naves P. et al. Measurement of biofilm formation by clinical isolates of Escherichia coli is method-dependent // J. Appl. Microbiol. 2008. Vol. 105. P. 585-590.
Pittman M.S. et al. Cysteine is exported from the Escherichia coli cytoplasm by CydDC, an ATP-binding cassette-type transporter required for cytochrome assembly // J. Biol. Chem. 2002. Vol. 277. P. 49841-49849.
Potamitou A., Holmgren A., Vlamis-Gardikas A. Protein levels of Escherichia coli thioredoxins and glutaredoxins and their relation to null mutants, growth phase, and function // J. Biol. Chem. 2002. Vol. 277. P. 18561-18567.
Povolotsky Т.Е., Hengge R. 'Life-style' control networks in Escherichia coli: Signaling by the second messenger c-di-GMP // J Biotechnol. 2012. Vol. 160. P. 10-16.
White-Ziegler C.A. et al. Low temperature (23°C) increases expression of biofilm-, cold-shock- and RpoS-dependent genes in Escherichia coli K-12 // Microbiology. 2008. Vol.154. P. 148-166.
References
Nilolaev Yu.A., Plakunov V.K. [Biofilm is «The town of microbes» or the analogue of a multicellular organism?] Microbiologiya. V. 76 (2007): pp. 149-163. (InRuss.).
Oktyabrsky O.N., Smirnova G.V. [The redox regulation of cellular functions] Biochimiya. V. 72 (2007): pp. 158-174. (InRuss.).
Baba Т., Ara Т., Hasegawa M., Takai Y., Okumura Y., Baba M., Datsenko K.A., Tomita M., Wanner B.L., Mori H. Construction of Escherichia coli strain K-12 in-frame, single-gene knockout mutants: the Keio Collection. Mol. Biol. Syst. Biol. V. 2 (2006) :0008
Beloin C., Roux A., Ghigo J-M. Escherichia coli biofilms. Curr. Top Microbiol. Immunol. V. 322 (2008): pp. 249-289
Mah T.F., Pitts В., Pelloc В., Walker G.C., Stewart P.S., O-Toole G.A. A genetic basis for Pseudomonas aeruginosa biofilm antibiotic resistance. Nature. V. 426 (2003): pp. 306-310.
Malpica R. Franco В., Rodriguez C., Kwon O., Geor-gellis D. Identification of a quinone-sensitive redox switch in the ArcB sensor kinase. Proc. Natl. Acad. Sei. V. 101 (2004): pp. 13318-13323.
Mika F, Hengge R. Small Regulatory RNAs in the control of motility and biofilm Formation in E. coli and Salmonella. Int. J. Mol. Sei. V. 14 (2013): pp. 4560-4579.
Miller J. N. Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory Press, 1972.
Naves P., del Prado G., Huelves L., Gracia M., Ruiz V., Blanco J., Rodriguez-Cerrato V., Ponte M.C., Soriano F. Measurement of biofilm formation by clinical isolates of Escherichia coli is method-dependent. J. Appl. Microbiol. V. 105 (2008): pp. 585-590.
Pittman M.S., Corker H., Wu G., Binet M.B., Moir A.J., Poole R.K. Cysteine is exported from the Escherichia coli cytoplasm by CydDC, an ATP-binding cassette-type transporter required for cytochrome assembly. J. Biol. Chem. V. 277 (2002): pp. 49841-49849.
Potamitou A., Holmgren A., Vlamis-Gardikas A. Protein levels of Escherichia coli thioredoxins and glutaredoxins and their relation to null mutants, growth phase, and function. J. Biol. Chem. V. 277 (2002): pp. 18561-18567.
Povolotsky T.L., Hengge R. 'Life-style' control networks in Escherichia coli: Signaling by the second messenger c-di-GMP. J Biotechnol. V. 160 (2012): pp. 10-16.
White-Ziegler C.A. Um S., Perez N., Berns A.L., Malhowski A.J., Young S. Low temperature (23 °C) increases expression of biofilm-, cold-shock- and RpoS-dependent genes in Escherichia coli K-12. Microbiology. V. 154 (2008): pp. 148-166.
