Differentiated impact assessment of the biogenic polyamines and alarmone synthetase RelMsm on the sliding motility of Mycobacterium smegmatis

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Published: Dec 24, 2024
DOI: https://doi.org/10.17072/1994-9952-2024-4-401-411
Keywords:
sliding motility bacterial spreading polyamines alarmone stress response

Main Article Content

Ivan V. Tsyganov
Institute of Ecology and Genetics of Microorganisms of the Perm Federal Research Center of Ural Branch RAS
Larisa Yu. Nesterova
Institute of Ecology and Genetics of Microorganisms of the Perm Federal Research Center of Ural Branch RAS
Alexander G. Tkachenko
Institute of Ecology and Genetics of Microorganisms of the Perm Federal Research Center of Ural Branch RAS

Abstract

The present article is devoted to the method for comparative analysis of sliding colonies. This new method includes measuring the density of bacterial cells in a monolayer. Using this method, we studied the effect of biogenic polyamines and alarmone synthetase RelMsm on Mycobacterium smegmatis sliding motility and showed a multidirectional effect of spermidine and spermine on sliding, as well as an increase in sliding motility by a strain with a relMsm deletion. It is also shown that there is no dependence between the sliding of mycobacteria and their hydrophobicity.

Article Details

How to Cite
Tsyganov И. В., Nesterova Л. Ю., & Tkachenko А. Г. (2024). Differentiated impact assessment of the biogenic polyamines and alarmone synthetase RelMsm on the sliding motility of Mycobacterium smegmatis. Bulletin of Perm University. Biology, (4), 401–411. https://doi.org/10.17072/1994-9952-2024-4-401-411
Issue
No. 4 (2024): Bulletin of Perm University. BIOLOGY
Section
Микробиология

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Author Biographies

Ivan V. Tsyganov, Institute of Ecology and Genetics of Microorganisms of the Perm Federal Research Center of Ural Branch RAS

Engineer of the Laboratory of Adaptation of Microorganisms, Junior Researcher of the Laboratory of Organic Synthesis

Larisa Yu. Nesterova, Institute of Ecology and Genetics of Microorganisms of the Perm Federal Research Center of Ural Branch RAS

Candidate of Biology, Senior Scientist of the Laboratory of Adaptation of Microorganisms, Associate Professor, Department of Plant Physiology and Soil Ecology

Alexander G. Tkachenko , Institute of Ecology and Genetics of Microorganisms of the Perm Federal Research Center of Ural Branch RAS

Doctor of Medicine, Head of the Laboratory of Adaptation of Microorganisms, professor of the Department of microbiology and immunology

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