The effect of heavy metals on strains of the genus Enterococcus The effect of heavy metals on strains of the genus Enterococcus

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Published: Dec 24, 2024
DOI: https://doi.org/10.17072/1994-9952-2024-4-390-400
Keywords:
genus Enterococcus heavy metals copper cadmium zinc

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Sergeevna
Far Eastern Federal University, Vladivostok, Russia
Viktorovna
Far Eastern Federal University, Vladivostok, Russia

Abstract

Heavy metals are widespread pollutants of natural water, where they end up because of almost constant use in agriculture and due to the discharge of industrial wastewater: primarily lead, cadmium and copper. On the other hand, heavy metals such as copper and zinc are known to be cofactors in many cellular processes and are required for cell survival in small concentrations. At the same time, such a heavy metal as cadmium does not function as a cofactor in the bacterial cell and has a toxic nature, disrupting cellular processes and causing cell death if the concentration exceeds a certain threshold. Thus, the sensitivity of enterococci to heavy metals is a property that characterizes the dynamics of anthropogenic load on the microbiocenosis of both freshwater and marine aquatic ecosystems. The aim of this investigation is to study the effect of heavy metals on strains of the genus Enterococcus isolated from the aquatic environment. Materials and methods: 18 water samples from the Second River mouth were studied, 30 strains of bacteria of the genus Enterococcus were isolated. Identification was carried out using the polymerase chain reaction method. Cell morphology was studied using light microscopy, and resistance to heavy metals was determined by the presence or absence of growth on a plate with different concentrations of metals (copper, cadmium, zinc). The results of the study showed that with an increase in the concentration of heavy metals in bacteria of the genus Enterococcus, the morphological characteristics change, and exceeding the MPC (maximum permissible concentration) leads to the death of the bacterium. According to the rate of action of the studied metals, they can be arranged in the following sequence: Cd > Cu > Zn.

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How to Cite
Uskova С. С., & Martynova А. В. (2024). The effect of heavy metals on strains of the genus Enterococcus : The effect of heavy metals on strains of the genus Enterococcus. Bulletin of Perm University. Biology, (4), 390–400. https://doi.org/10.17072/1994-9952-2024-4-390-400
Issue
No. 4 (2024): Bulletin of Perm University. BIOLOGY
Section
Микробиология

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Author Biographies

Sergeevna, Far Eastern Federal University, Vladivostok, Russia

postgraduate student of the department of biodiversity and marine bioresources

Viktorovna, Far Eastern Federal University, Vladivostok, Russia

doctor of medical sciences, professor of the department of biodiversity and marine bioresources; professor of the Department of Epidemiology and Military Epidemiology

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