Isolation and сharacterization of dibutyl phthalate-degrading strain Rhodococcus sp. 5A-K4

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Published: Nov 5, 2024
DOI: https://doi.org/10.17072/1994-9952-2024-3-309-317
Keywords:
Puccinellia distans rhizosphere dibutyl phthalate degradation Rhodococcus

Main Article Content

Anna A. Pyankova
Institute of Ecology and Genetics of Microorganisms, Perm, Russia
Alexandra V. Kraeva
Perm State University, Perm, Russia
Yulia L. Nechaeva
Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, RAS, Perm, Russia
Elena G. Plotnikova
Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, RAS, Perm, Russia

Abstract

The study is devoted to the ability of the bacterial strain 5A-K4, isolated from the rhizosphere of Puccinellia distans (Jacq.) Parl. plants, growing on the territory of industrial development of the Verkhnekamsk salt deposit (Perm krai), to grow on dibutyl phthalate (DBP) as the single carbon source was studied. Based on analysis of the 16S rRNA gene, the strain was identified as a representative of the genus Rhodococcus. Strain 5A-K4 had the highest level of the 16S rRNA gene similarity (99.86%) with Rhodococcus erythropolis NBRC 15567T. The strain grew effectively on a DBP substrate without addition of salt and at a content of 30 g/L NaCl in the cultivation medium. It has been shown that Rhodococcus sp. 5A-K4 is capable of growth at high concentrations of DBP (up to 12 g/L). The genome of the strain 5A-K4 contained the dpeH and mpeH genes, the products of which are involved in the initial stages of DBP degradation. The nucleotide sequences of the dpeH and mpeH genes are homologous to the sequences of the α/β hydrolase genes of strains of the classes Actinomycetes and Bacilli. Based on the data obtained, a pathway for the degradation of DBP by strain 5A-K4 was proposed. Thus, the rhizosphere DBP degrading strain Rhodococcus sp. 5A-K4 can be used as a bacterial agent in the development of phytoremediation methods for soils contaminated with phthalates.

Article Details

How to Cite
Pyankova А. А., Kraeva А. В. ., Nechaeva Ю. И. ., & Plotnikova Е. Г. . (2024). Isolation and сharacterization of dibutyl phthalate-degrading strain Rhodococcus sp. 5A-K4. Bulletin of Perm University. Biology, (3), 309‒317. https://doi.org/10.17072/1994-9952-2024-3-309-317
Issue
No. 3 (2024): Bulletin of Perm University. BIOLOGY
Section
Микробиология

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Author Biographies

Anna A. Pyankova, Institute of Ecology and Genetics of Microorganisms, Perm, Russia

junior researcher

Alexandra V. Kraeva, Perm State University, Perm, Russia

student

Yulia L. Nechaeva, Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, RAS, Perm, Russia

engineer IEGM UB RAS, postgraduate student PSU

Elena G. Plotnikova, Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, RAS, Perm, Russia

doctor of biological sciences, associate professor, head of laboratory IEGM UB RAS, professor PSU

References

Бачурин Б.А., Одинцова Т.А. Стойкие органические загрязнители в отходах горного производства // Современные экологические проблемы Севера. Апатиты: Изд-во Кольского НЦ РАН, 2006. Ч. 2. С. 7–9.

Нетрусов А.И. Практикум по микробиологии. М.: Академия, 2005. 608 с.

Чернявская М.И. и др. Первичный анализ генома бактерий-деструкторов нефти Rhodococcus pyridinivorans 5Ap // Труды БГУ. 2016. Т. 11, ч. 1. С. 219–223.

Aleshchenkova Z.M. et al. The degradation of plasticizers by Rhodococcus erythropolis 40F // Mikrobiol. Z. 1996. Vol. 58(4). P. 34–38.

Ausbel F.M. Short Protocols in Molecular Biology. 3rd ed. N.Y.: John Wiley & Sons, 1995. 450 p.

Chao W.L., Cheng C.-Y. Effect of introduced phthalate-degrading bacteria on the diversity of indigenous bacterial communities during di-(2-ethylhexyl) phthalate (DEHP) degradation in a soil microcosm // Chemo-sphere. 2007. Vol. 67(3). P. 482–488.

Chao W.L. et al. Degradation of di-butyl-phthalate by soil bacteria // Chemosphere. 2006. Vol. 63(8). P. 1377–1383.

Gao D.-W., Wen Z.-D. Phthalate esters in the environment: a critical review of their occurrence, biodegra-dation, and removal during wastewater treatment processes // Science of the Total Environment. 2016. Vol. 541. P. 986–1001.

Gardner S.T. et al. Assessing differences in toxicity and teratogenicity of three phthalates, diethyl phthalate, di-n-propyl phthalate, and di-n-butyl phthalate, using Xenopus laevis embryos // Journal of Toxicol-ogy and Environmental Health. 2016. Vol. 79(2). P. 71–82.

He Z., Niu C., Lu Z. Individual or synchronous biodegradation of di-n-butyl phthalate and phenol by Rhodococcus ruber strain DP-2 // Journal of Hazardous Materials. 2014. Vol. 273. P. 104–109.

Jin D.-C. et al. Biodegradation of di-n-butyl phthalate by Rhodococcus sp. JDC-11 and molecular detec-tion of 3,4-phthalate dioxygenase gene // Journal of Microbiology and Biotechnology. 2010. Vol. 20(10) P. 1440–1445.

Lane D.J. 16S/23S rRNA sequencing // Nucleic acid techniques in bacterial systematics. 1991. P. 115–175.

Li J. et al. Bioremediation of environmental endocrine disruptor di-n-butyl phthalate ester by Rhodococ-cus ruber // Chemosphere. 2006. Vol. 65(9). P. 1627–1633

Li K., Gu J. Biodegradation of di-n-butyl phthalate by mangrove microorganism Rhodococcus ruber 1K // Ying Yong Sheng Tai Xue Bao. 2005. Vol. 16(8). P. 1566–1574.

Liu T. et al. Synthetic bacterial consortia enhanced the degradation of mixed priority phthalate ester pol-lutants // Environmental Research. 2023. Vol. 235. P. 116–121.

Lu M. et al. Degradation of dibutyl phthalate (DBP) by a bacterial consortium and characterization of two novel esterases capable of hydrolyzing PAEs sequentially // Ecotoxicology and Environmental Safety. 2020. Vol. 195. P. 1–9.

Lu Y. et al. Biodegradation of dimethyl phthalate, diethyl phthalate and di-n-butyl phthalate by Rhodo-coccus sp. L4 isolated from activated sludge // Journal of Hazardous Materials. 2009. Vol. 168(2-3). P. 938–943.

Mahajan R. et al. Biodegradation of di-n-butyl phthalate by psychrotolerant Sphingobium yanoikuyae strain P4 and protein structural analysis of carboxylesterase involved in the pathway // International Journal of Biological Macromolecules. 2019. Vol. 122. P. 806–816.

Nazarov A.V. et al. Soil bacterial communities in the affected zone of salt dump (Solikamsk, Perm krai) // Eurasian Soil Science, 2024, Vol. 57(8). P. 1353–1361.

Raymond R.L. Microbial oxidation of n-paraffinic hydrocarbons // Developments in Industrial Microbi-ology. 1961. Vol. 2(1). P. 23–32.

Song X. et al. Biodegradation of phthalate acid esters by a versatile PAE-degrading strain Rhodococcus sp. LW-XY12 and associated genomic analysis // International Biodeterioration and Biodegradation. 2022. Vol. 170. P. 1–12.

Vamsee-Krishna C., Phale P.S. Bacterial degradation of phthalate isomers and their esters // Indian Jour-nal of Microbiology. 2008. Vol. 48. P. 19–34.

Wang L. et al. Analysis of the performance of the efficient di-(2-ethylhexyl) phthalate-degrading bacte-rium Rhodococcus pyridinovorans DNHP-S2 and associated catabolic pathways // Chemosphere. 2022. Vol. 306. P. 135–142.

Yang T. et al. Biodegradation of di-(2-ethylhexyl) phthalate by Rhodococcus ruber YC-YT1 in contami-nated water and soil // International Journal of Environmental Research and Public Health. 2018. Vol. 15(5). P. 1–20.