MicroRNA expression profiles in blood leukocytes as autism spectrum disorder severity markers in children

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Published: Nov 5, 2024
DOI: https://doi.org/10.17072/1994-9952-2024-3-335-343
Keywords:
microRNAs miR-146a leukocytes cytokines autism spectrum disorder child diagnosis

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Anna S. Alekseeva
Chelyabinsk State University, Chelyabinsk, Russia
Yuliya Yu. Filippova
Chelyabinsk State University, Chelyabinsk, Russia
Aleksandra L. Burmistrova
Chelyabinsk State University, Chelyabinsk, Russia

Abstract

Autism spectrum disorders are a heterogeneous group of neurodevelopmental disorders with unknown etiology. Immune dysfunction may be involved in the etiology and pathogenesis of autism. One of the regulators of interactions between the nervous and immune systems are microRNAs, which can be considered as key players in pathogenesis and diagnostic biomarkers. The present study is devoted to a search for the relationship between leukocyte expression of microRNAs: microRNA-155, microRNA-146a, microRNA-124, microRNA-21 and microRNA-9, and the concentration of cytokines in the blood plasma of autistic children, which was carried out to detect biological markers of the disease and its severity. It was found that in children with mild autism, the expression levels of the studied microRNAs in blood leukocytes did not differ from similar values in children with normotypical development. In leukocytes of children with severe autism spectrum disorders, the expression of microRNA-124 and microRNA-146a is reduced compared to normotypical children, and microRNA-146a is reduced compared to children with mild autism.  Two significant positive correlations between microRNA-9/IFNg and microRNA-124/TNFa against the background of a negative interaction between microRNA-155 and TNFa were identified in the group of children with mild autism.  Four significant negative relationships between microRNA-9/IFNg; microRNA-146a/IFNg; miR-146a/IL-6 and miR-155/IL-10 were found in children with severe autism spectrum disorders. The expression level of microRNA-146a in leukocytes less than 0.0035 с.u. with 86.7% sensitivity and 89.6% specificity may indicate severe autism in children. Thus, we have identified disturbances in the expression levels of important negative regulators of inflammation and participants in neuro-immune interactions – microRNA-146a and microRNA-124.

Article Details

How to Cite
Alekseeva А. С. ., Filippova Ю. Ю. ., & Burmistrova А. Л. . (2024). MicroRNA expression profiles in blood leukocytes as autism spectrum disorder severity markers in children. Bulletin of Perm University. Biology, (3), 335‒343. https://doi.org/10.17072/1994-9952-2024-3-335-343
Issue
No. 3 (2024): Bulletin of Perm University. BIOLOGY
Section
Иммунология

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Author Biographies

Anna S. Alekseeva, Chelyabinsk State University, Chelyabinsk, Russia

Assistant, Department of Microbiology, Immunology and General Biology, Faculty of Biology «Chelyabinsk State University»

Yuliya Yu. Filippova, Chelyabinsk State University, Chelyabinsk, Russia

PhD (Biology), Professor, Department of Microbiology, Immunology and General biology, Faculty of Biology, Chelyabinsk State University

Aleksandra L. Burmistrova, Chelyabinsk State University, Chelyabinsk, Russia

PhD, MD (Medicine), Professor, Head, Department of Microbiology, Immunology and General Biology, Faculty of Biology, Chelyabinsk State University

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