Comparative analysis of the biodiversity of endophytic microorganisms of spring durum wheat at different phases of plant growth and development

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Published: Jul 21, 2024
DOI: https://doi.org/10.17072/1994-9952-2024-2-165-175
Keywords:
Triticum durum spring durum wheat endophytic microorganisms phenological phases

Main Article Content

Alexey R. Dolgov
Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia
Denis V. Utkin
Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia
https://orcid.org/0000-0002-5903-7700
Eduard G. Khachaturov
Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia
https://orcid.org/0000-0002-4391-8909
Dmitry A. Polivanov
Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia
Natalia E. Shcherbakova
Russian Anti-plague Institute "Microbe" of Rospotrebnadzor, Saratov, Russia
Maxim V. Karlov
Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia
Mikhail Y. Kasatkin
Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia
Sergey A. Stepanov
Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia
Galina I. Shutareva
Federal Agrarian Scientific Center of the South-East, Saratov, Russia
https://orcid.org/0000-0003-1159-2892

Abstract

The rapid expansion of knowledge about the interrelationships of microorganisms with plants creates an opportunity for the transition to environmentally sustainable farming systems. The interaction of microorganisms with higher plants creates the basis for the development of crop production industries, providing humanity with environmentally friendly food products. The purpose of the research is to study the biodiversity of the species composition of endophytic microorganisms of spring durum wheat isolated in different phases of plant growth and development. In the work, seeds and plants were used at different phases of vegetation of varieties of Saratov selection: Saratov golden, Ray 25 and Vasilchuk Memory. Sampling of plants from April to June 2023 was carried out from the variety testing site of the Federal Agrarian Scientific Center of the South-East. Endophytic microorganisms were isolated from seeds and plant organs (roots, leaves, stems, ears) after surface sterilization and homogenization. The identification of isolated microorganisms was carried out by bacteriological methods and using MALDI ToF mass spectrometry. As a result of the conducted studies, 30 strains of bacteria belonging to the genera Pantoea, Enterobacter, Citrobacter, Pseudomonas, Bacillus and Staphylococcus were identified. A change in the species composition of microorganisms in the process of plant ontogenesis was noted, associated with activation, colonization and selective advantage of individual species of microorganisms. The isolated strains of endophytic microorganisms are promising for the creation of biological products to increase plant resistance to abiotic and biotic factors.

Article Details

How to Cite
Dolgov А. Р., Utkin Д. В., Khachaturov Э. Г., Polivanov Д. А., Shcherbakova Н. Е., Karlov М. В., Kasatkin М. Ю., Stepanov С. А., & Shutareva Г. И. (2024). Comparative analysis of the biodiversity of endophytic microorganisms of spring durum wheat at different phases of plant growth and development. Bulletin of Perm University. Biology, (2), 165–175. https://doi.org/10.17072/1994-9952-2024-2-165-175
Issue
No. 2 (2024): Bulletin of Perm University. BIOLOGY
Section
Микробиология

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Author Biographies

Alexey R. Dolgov, Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

Bachelor of the Faculty of Biology

Denis V. Utkin , Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

Doctor of Biological Sciences, Professor of the Department of Microbiology and Plant Physiology

Eduard G. Khachaturov, Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

Assistant of the Department of Microbiology and Plant Physiology

Dmitry A. Polivanov, Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

Bachelor of the Faculty of Biology

Natalia E. Shcherbakova, Russian Anti-plague Institute "Microbe" of Rospotrebnadzor, Saratov, Russia

Researcher of the Department of Diagnostics of Infectious Diseases

Maxim V. Karlov, Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

Bachelor of Biological Faculty

Mikhail Y. Kasatkin, Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

Candidate of Biological Sciences, Associate Professor, Associate Professor of the Department of Microbiology and Plant Physiology

Sergey A. Stepanov, Saratov National Research State University named after N.G. Chernyshevsky, Saratov, Russia

Doctor of Biological Sciences, Professor, Head of the Department of Microbiology and Plant Physiology

Galina I. Shutareva, Federal Agrarian Scientific Center of the South-East, Saratov, Russia

Head of the Laboratory of Breeding and seed production of spring durum wheat

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