Dibutyl phthalate degrading bacteria isolated from the rhizosphere of bluegrass (Poa pratensis L.)

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Published: Dec 15, 2023
DOI: https://doi.org/10.17072/1994-9952-2023-4-349-355
Keywords:
Poa pratensis rhizosphere dibutyl phthalate degradation Rhodococcus Pseudoarthrobacter

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Ekaterina S. Korsakova
Institute of Ecology and Genetics of Microorganisms, Perm State University, Perm, Russia
Anna A. Pyankova
Institute of Ecology and Genetics of Microorganisms, Perm, Russia
Elena G. Plotnikova
Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the RAS, Perm, Russia

Abstract

The ability of three bacterial strains of the class Actinomycetes, isolated from the rhizosphere of bluegrass (Poa pratensis L.) plants growing in the industrial development area of the Verkhnekamsk salt deposit (Perm krai), to grow on dibutyl phthalate (DBP) as the only source of carbon and energy was studied. Based on 16S rRNA gene analysis, it was shown that the strain Rh7bel showed similarity at 100% level with Rhodococcus wratislaviensis NBRC 100605T, and strains NKDBFbel and NKDBFgelt are phylogenetically close to two type strains of the species Pseudarthrobacter oxydans and Pseudarthrobacter polychromogenes (99.83% similarity). DBP-degrading strains are characterized by efficient growth on the key metabolite of DBP degradation, ortho-phthalic acid, and utilization of this metabolite. The strain Rhodococcus sp. Rh7bel demonstrated the highest rates of DBP utilization: the maximum specific substrate consumption rate was 0.018±0.002 h-1, substrate utilization was 70.7% in 72 hours (initial DBP concentration 0.2 g/L). Thus, rhizosphere DBP-degrading strains Rhodococcus sp. Rh7bel, Pseudarthrobacter spp. NKDBFbel and NKDBFgelt are promising for further study and development of technology for phytoremediation of soils contaminated with phthalates.

Article Details

How to Cite
Korsakova Е. С., Pyankova А. А., & Plotnikova Е. Г. (2023). Dibutyl phthalate degrading bacteria isolated from the rhizosphere of bluegrass (Poa pratensis L.). Bulletin of Perm University. Biology, (4), 349–355. https://doi.org/10.17072/1994-9952-2023-4-349-355
Issue
No. 4 (2023): Bulletin of Perm University. BIOLOGY
Section
Микробиология

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Author Biographies

Ekaterina S. Korsakova, Institute of Ecology and Genetics of Microorganisms, Perm State University, Perm, Russia

Candidate of biological sciences, researcher, associate professor of the Department of Botany and Plant Genetics

Anna A. Pyankova, Institute of Ecology and Genetics of Microorganisms, Perm, Russia

Junior researcher

Elena G. Plotnikova, Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the RAS, Perm, Russia

Doctor of biological sciences, associate professor, head of laboratory, professor of the Department of Botany and Plant Genetics

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