Solid-phase enzyme-linked immunosorbent assay (direct option) for detecting toxigenic vibrio cholerae serogroups О1 and О139

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Published: Dec 23, 2022
DOI: https://doi.org/10.17072/1994-9952-2022-4-280-287
Keywords:
cholera toxin polyclonal and monoclonal peroxidase conjugates enzyme immunoassay laboratory diagnosis of cholera

Main Article Content

Lyudmila P. Alekseeva
Rostov-on-Don Anti-Plague Institute, Rostov-on-Don, Russia
https://orcid.org/0000-0002-9866-3579
Olga A. Yakusheva
Rostov-on-Don Anti-Plague Institute, Rostov-on-Don, Russia
https://orcid.org/0000-0001-8159-7547
Veronika V. Evdokimova
Rostov-on-Don Anti-Plague Institute, Rostov-on-Don, Russia
https://orcid.org/0000-0001-5522-9097
Mikhail E. Yagovkin
Rostov-on-Don Anti-Plague Institute, Rostov-on-Don, Russia
https://orcid.org/0000-0001-8414-4965
Vera P. Zyuzina
Rostov-on-Don Anti-Plague Institute, Rostov-on-Don, Russia
https://orcid.org/0000-0003-3100-0049

Abstract

As a result of the studies, the method of direct solid-phase enzyme immunoassay (ELISA test) was optimized for detecting toxigenic strains of Vibrio cholerae O1 and O139 serogroups based on monoclonal and polyclonal antitoxic immunoglobulin peroxidase conjugates. The study detected the following optimal parameters for the analysis: sensitization of a tablet by toxicodermia drugs diluted in phosphate-saline buffer (PBS) pH 7.4, up to titers of 1:2-1:4, their adsorption on the solid phase of the tablet for 2 hours at 37° C, blocking of free binding sites by the 1% solution of bovine serum albumin (BSA) for 30 minutes (at 37°C); contact time of the investigated sample with mono- or polyclonal conjugates peroxidase - 30 minutes (at 37°C). Tests on homologous and heterologous strains showed a high specificity of the enzyme immunoassay and the possibility of its use for detecting toxigenic Vibrio cholerae.

Article Details

How to Cite
Alekseeva Л. П. ., Yakusheva О. А. ., Evdokimova В. В. ., Yagovkin М. Э. ., & Zyuzina В. П. . (2022). Solid-phase enzyme-linked immunosorbent assay (direct option) for detecting toxigenic vibrio cholerae serogroups О1 and О139. Bulletin of Perm University. Biology, (4), 280–287. https://doi.org/10.17072/1994-9952-2022-4-280-287
Issue
No. 4 (2022): Bulletin of Perm University. BIOLOGY
Section
Микробиология

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Author Biographies

Lyudmila P. Alekseeva, Rostov-on-Don Anti-Plague Institute, Rostov-on-Don, Russia

Doctor of biological sciences, professor

Olga A. Yakusheva, Rostov-on-Don Anti-Plague Institute, Rostov-on-Don, Russia

Research associate

Veronika V. Evdokimova , Rostov-on-Don Anti-Plague Institute, Rostov-on-Don, Russia

Candidate of biological sciences, researcher

Mikhail E. Yagovkin, Rostov-on-Don Anti-Plague Institute, Rostov-on-Don, Russia

Research associate

Vera P. Zyuzina, Rostov-on-Don Anti-Plague Institute, Rostov-on-Don, Russia

Candidate of biological sciences, senior researcher

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