Phylogenetic diversity of bacteria in rock salt of the Verkhnekamskoe deposit (Perm Krai)

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Published: Dec 24, 2021
DOI: https://doi.org/10.17072/1994-9952-2021-4-256-262
Keywords:
Verkhnekamskoe salt deposit unculturable bacteria cloning DNA sequencing 16S rRNA gene

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Anna A. Pyankova
Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS
https://orcid.org/0000-0003-2210-783X
Yulia A. Beloglazova
Institute of Technical Chemistry
https://orcid.org/0000-0003-1141-6266
Ekaterina S. Korsakova
Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS
https://orcid.org/0000-0002-6907-7562
Boris A. Bachurin
Mining Institute, Ural Branch of RAS
https://orcid.org/0000-0001-7200-2986
Elena G. Plotnikova
Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS
https://orcid.org/0000-0002-0107-0719

Abstract

New data on the phylogenetic diversity of bacteria in the salt of the Verkhnekamskoe deposit (Perm Krai) have been obtained using molecular genetic methods. Analysis of the 16S rRNA genes of total DNA from the rock salt sample (depth intervals of 239.7-239 m) evinces the presence of bacteria belonging to the classes: Actinobacteria (closely related to the genera Rhodococcus, Demequina), Gammaproteobacteria (the genera Pseudomonas, Serratia, Shigella), Betaproteobacteria (the genus Ralstonia) and Alphaproteobacteria (the genus Phyllobacterium). In addition, we identified two phylotypes of the Alphaproteobacteria (clone 66BA (GenBank MH410136) and clone 12BA (GenBank MH410128) that show lower similarity in 16S rRNA genes (98.46%) with the closest type strains of the genus Mesorhizobium (M. alhagi CCNWXJ12-2T) and the genus Chelativorans (C. multitrophicus DSM9103T). The identified phylotypes may represent new taxonomic units.

Article Details

How to Cite
Pyankova А. А., Beloglazova Ю. А., Korsakova Е. С., Bachurin Б. А., & Plotnikova Е. Г. (2021). Phylogenetic diversity of bacteria in rock salt of the Verkhnekamskoe deposit (Perm Krai). Bulletin of Perm University. Biology, (4), 256–262. https://doi.org/10.17072/1994-9952-2021-4-256-262
Issue
No. 4 (2021): Bulletin of Perm University. Biology
Section
Микробиология

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Author Biographies

Anna A. Pyankova, Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS

Junior researcher

Yulia A. Beloglazova, Institute of Technical Chemistry

Junior researcher

Ekaterina S. Korsakova, Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS

Candidate of biology

Boris A. Bachurin, Mining Institute, Ural Branch of RAS

Candidate of geological and mineralogy

Elena G. Plotnikova, Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS

Doctor of biology, professor

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