Morphological aspects of adaptation of the alkalophilic bacteria Bacillus aequororis to high salinity and alkalinity of the medium

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Published: Dec 7, 2021
DOI: https://doi.org/10.17072/1994-9952-2021-3-178-184
Keywords:
alkalophiles bacilli atomic force microscopy morphology

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Anna V. Shilova
Institute of Ecology and Genetics of Microorganisms UB RAS
https://orcid.org/0000-0001-8964-7502
Grigory G. Glebov
Institute of Ecology and Genetics of Microorganisms UB RAS
https://orcid.org/0000-0001-7220-4553
Yuliya G. Maksimova
Institute of Ecology and Genetics of Microorganisms UB RAS
https://orcid.org/0000-0003-1870-1369

Abstract

AFM-images were obtained and the morphometric parameters of cells of the facultative alkalophile Bacillus aequororis 5-DB and neutrophilic Bacillus subtilis ATCC 6633 were calculated after incubation in an alkaline medium with a high concentration of sodium chloride. It was shown that adaptation of B. aequororis 5-DB is not accompanied by significant changes in morphometric parameters, while the volume of cells slightly (by 1–1.5 times) decreases and their surface roughness increases. Neutrophilic bacillus, on the contrary, reacts to an increase in salt concentration and pH (50 g/l, pH 11) by significant changes in the cell: the cell volume decreases 2–3 times, and with daily adaptation to 50 g/l salt with pH 8 increases by 2.4 times due to the increase in cell length. Cells of an alkalophilic bacillus, in contrast to those of a neutrophilic bacillus, do not undergo significant changes in morphology with a high mineralization of the environment, both under direct exposure and after daily adaptation.

Article Details

How to Cite
Shilova А. В., Glebov Г. Г., & Maksimova Ю. Г. (2021). Morphological aspects of adaptation of the alkalophilic bacteria Bacillus aequororis to high salinity and alkalinity of the medium. Bulletin of Perm University. Biology, (3), 178–184. https://doi.org/10.17072/1994-9952-2021-3-178-184
Issue
No. 3 (2021): Bulletin of Perm University. Biology
Section
Микробиология

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Author Biographies

Anna V. Shilova, Institute of Ecology and Genetics of Microorganisms UB RAS

Graduate student

Grigory G. Glebov, Institute of Ecology and Genetics of Microorganisms UB RAS

Graduate student

Yuliya G. Maksimova, Institute of Ecology and Genetics of Microorganisms UB RAS

Doctor of biology, associate professor, Head of the Laboratory of Molecular Biotechnology

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