Association of Ser447Ter polymorphisms of the LPL gene and rs9939609 of the FTO gene with obesity in children and adolescents in the Rostov-on-Don population

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Published: Nov 8, 2021
DOI: https://doi.org/10.17072/1994-9952-2021-2-119-127
Keywords:
LPL Ser447Ter FTO rs9939609 children and adolescents obesity

Main Article Content

Abd Ali Alaa Hashim
Southern Federal University
https://orcid.org/0000-0002-9664-7751
Olga Bocharova
Rostov State Medical University
https://orcid.org/0000-0002-5056-1506
Tatyana Shkurat
Southern Federal University
https://orcid.org/0000-0001-6197-7374
Mikhail Shkurat
Limited liability company «Science»
https://orcid.org/0000-0002-9383-4607
Maria Amelina
Southern Federal University
https://orcid.org/0000-0002-7459-2796

Abstract

The aim of this study was to study the correlation between the Ser447ter (C-G) polymorphisms of the LPL gene and rs9939609 of the FTO gene and obesity in children and adolescents of the Rostov region (Russia). A case-control study examined the relationship between the rs9939609 polymorphisms of the FTO gene and Ser447Ter (S447X) of the LPL gene with obesity in 520 children and adolescents of both sexes aged 3 to 18 years: the main group consisted of 370 obese children and adolescents, and the control group - 150 children and adolescents without obesity. Genotyping of polymorphisms T / A rs9939609 of the FTO gene and C / G Ser447Ter of the LPL gene was performed using PCR- allele-specific primers. Polymorphisms of the FTO rs9939609 and LPL Ser447Ter genes in donor DNA samples were typed by the electrophoretic method using commercial test systems from the Litekh research and production company (Russia). The relationship between the rs9939609 polymorphism of the FTO gene and obesity was revealed, differences (P<0.05) were established between the main and control groups in the frequency of occurrence of the AA genotype (P = 0.0079) and allele A (P = 0.005; OR 0.67; 95% CI 0.51–0.88) of the rs9939609 polymorphism of the FTO gene. Combinations of genotypes with increased and decreased risk of obesity in the child and adolescent population of Rostov-on-Don were determined. Antagonistic relationships between the rs9939609 polymorphisms of the FTO gene and Ser447Ter of the LPL gene are shown. Using the MDR method, combinations of genotypes with an increased and decreased risk of obesity in the child and adolescent population of Rostov-on-Don were established.

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How to Cite
Hashim А. А. А. ., Bocharova О. В., Shkurat Т. П., Shkurat М. А., & Amelina М. А. (2021). Association of Ser447Ter polymorphisms of the LPL gene and rs9939609 of the FTO gene with obesity in children and adolescents in the Rostov-on-Don population. Bulletin of Perm University. Biology, (2), 119–127. https://doi.org/10.17072/1994-9952-2021-2-119-127
Issue
No. 2 (2021): Bulletin of Perm University. Biology
Section
Генетика

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Author Biographies

Abd Ali Alaa Hashim, Southern Federal University

PhD student

Olga Bocharova , Rostov State Medical University

PhD student

Tatyana Shkurat , Southern Federal University

Doctor of Biological Sci­ences, Professor, Head of the Department of Genetics

Mikhail Shkurat , Limited liability company «Science»

Senior Researcher

Maria Amelina , Southern Federal University

Senior Researcher

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