MOLECULAR GENETIC IDENTIFICATION OF POPULATIONS OF PINUS SYLVESTRYS L. IN THE EAST OF THE RUSSIAN PLAIN BASED ON POLYMORPHISM ISSR-MARKERS

Article Sidebar

PDF (Русский)
Published: Oct 23, 2018
Keywords:
dna polymorphism molecular genetic identification pinus sylvestris l issr-markers

Main Article Content

Яна/Yana Викторовна/Viktorovna Пришнивская/Prishnivskaya
Perm State University; Natural Science Institute Perm State University
Виталий/Vitaliy Павлович/Pavlovich Красильников/Krasilnikov
Perm State University
Светлана/Svetlana Витальевна/Vitalievna Боронникова/Boronnikova
Perm State University

Abstract

A molecular genetic analysis and identification of the four populations of Pinus sylvestris L., located in the Komi Republic and Kirov region, using ISSR-method of DNA polymorphism analysis. In four studied populations P. sylvestris identified 117 ISSR-markers; a high percentage of polymorphic loci (Р95 = 0.949). One ISSR-primer initiated synthesis in P. sylvestris on average 16.7 ISSR-markers. In the studied populations of P. sylvestris identified 16 rare ISSR-markers. Revealed the identity of the monomorphic species and polymorphic ISSR-markers, and combinations there of for the molecular genetic identification of the populations studied. Compiled by molecular genetic formula and barcodes four studied populations of P. sylvestris. The data obtained can be used to identify populations and wood of P. sylvestris in the studied region.

Article Details

How to Cite
Пришнивская/Prishnivskaya Я. В., Красильников/Krasilnikov В. П., & Боронникова/Boronnikova С. В. (2018). MOLECULAR GENETIC IDENTIFICATION OF POPULATIONS OF PINUS SYLVESTRYS L. IN THE EAST OF THE RUSSIAN PLAIN BASED ON POLYMORPHISM ISSR-MARKERS. Bulletin of Perm University. Biology, (2), 171–176. Retrieved from http://press.psu.ru/index.php/bio/article/view/1805
Issue
No. 2 (2016): Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology
Section
Генетика

License agreement

 

Author Biographies

Яна/Yana Викторовна/Viktorovna Пришнивская/Prishnivskaya, Perm State University; Natural Science Institute Perm State University

Student of the Department of Botany and Plant Genetics;Engineer-researcher of the laboratory of molecular biology, and finally genetics

Виталий/Vitaliy Павлович/Pavlovich Красильников/Krasilnikov, Perm State University

Graduate student faculty of Biology

Светлана/Svetlana Витальевна/Vitalievna Боронникова/Boronnikova, Perm State University

Doctor of Biological Sciences, Professor, Head of the Department of Botany and Plant Genetics

References

Боронникова C.B. Исследование генетической изменчивости популяций редкого вида Урала Adenophora lilifolia (L.) DC. на основании анализа полиморфизма ISSR-маркеров // Генетика. 2009. Т. 45, № 5. С. 652-655.

Боронникова C.B. Молекулярно-генетический анализ и оценка состояния генофондов ресурсных видов растений Пермского края: монография. Пермь, 2013. 223 с.

Боронникова C.B., Календарь Р.И. Использование IRAP-метода для анализа генетической изменчивости популяций ресурсных и редких видов растений // Генетика. 2010. Т. 46, № 1 С. 44-50.

Ветчинникова Л.В., Титов А. Ф., Кузнецова Т.Ю. Карельская береза: биологические особенности, динамика ресурсов и воспроизводство. Петрозаводск: Карельский научный центр РАН, 2013. 312 с.

Грант В. Видообразование у растений. М.: Мир, 1984. 528 с.

Исаев A.C., Коровин Г.И. Актуальные проблемы национальной лесной политики / Центр по проблемам экологии и продуктивности лесов Российской академии наук. М., 2009. 108 с.

Левонтин P.C. Генетические основы эволюции. М.: Мир, 1978. 351 с.

Материалы к заседанию "круглого стола" на тему «Законодательное обеспечение основных направлений развития лесного хозяйства на базе инновационных научно-технических достижений». 2012. URL: http://council.gov.ru/media/files/41d4935c0f740f5 ela2b.pdf (дата обращения: 03.04.2016).

Нечаева Ю.С. Оптимизация методики выделения ДНК некоторых хвойных видов растений Пермского края // Материалы международной конференции «Синтез знаний в естественных науках. Рудник будущего: проекты, технологии, оборудование». Пермь, 2011. С. 278-282.

Основные результаты работы Министерства природных ресурсов и экологии Российской Федерации за 2013 г. URL: http://government.ru/dep_news/11859/ (дата обращения: 02.04.2016).

Geburek Т., Turok J. Conservation and sustainable management of forest genetic resources in Europe - an introduction // Conservation and Management of Forest Genetic Resources in Europe. Ar-bora Publishers, Zvolen, 2005. P. 3-8.

Rogers S.O., Bendich A.J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues // Plant Molecular Biology. 1985. Vol. 1, № 19. P. 69-76.

Zietkiewicz E. Rafalski A., Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification // Genomics. 1994. Vol. 20. P. 176-183.

References

Boronnikova S.V. Molekuljarno-genetuceskij analiz i ocenka sostojanija genofondov resursnych vidov rastenij Permskogo kraja [Molecular genetic analysis and assessment of gene pools of resource species of plants of Perm region: monograph], Perm, 2013. 223 p. (In Russ.).

Boronnikova S.V. Genetic variation in Ural populations of the rare plant species Adenophora lilifolia (L.) D. on the basis of analysis of polymorphism of ISSR-markers. Russian Journal of Genetics. 2009, V. 45, N5, pp. 571-574.

Boronnikova S.V., Kalendar R.N. Using IRAP-markers for analisis of genetic variability in populations of resource and rare species of plants. Russian Jornal of Genetics. 2010, N 1 (46), pp. 36-42.

Geburek Т., Turok J. Conservation and sustainable management of forest genetic resources in Europe - an introduction. Conservation and Management of Forest Genetic Resources in Europe. Arbora Publishers, Zvolen, 2005, pp. 3-8.

Grant B. Vidoobrazovanie и rastenij [Speciation in plants], Moscow, Mir Publ., 1984. 528 p. (In Russ.).

Isaev A.S., Korovin G.N. Aktual'nye problemy national 'noj lesnoj politiki [Actual problems of the National Forest Policy]. Moscow, 2009. 108 p. (In Russ.).

LewontinR.S. Geneticeskie osnovy Svoljucii [Genetic bases of the evolution], Moscow, Mir Publ., 1978. 351 p. (In Russ.).

Materials for the meeting of the "round table" on "Legislative support of the main directions of forestry development based on innovative scientific and technological achievements." 2012. URL: http://council.gov.ru/media/files/41d4935c0f740f5ela2b.pdf (reference date 03/04/2016). (In Russ.).

Nechaeva Yu.S. [Optimization of DNA extraction methods of some coniferous species of plants of Perm region], Materialy mezdunarodnoj konfer-encii "Sintez znanij v estestvennych naukach. Rudnik buduscego: proekty, technologii, oborudo-vanie" [Proceedings of the international conference «Knowledge Synthesis in the natural sciences. Rudnik future: projects, technologies and equipment»]. Perm, 2011, pp. 278- 282. (In Russ.).

Rogers S.O., Bendich A.J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Molecular Biology, 1985, V. 1, N 19, pp. 69-76.

The main results of the Ministry of Natural Resources and Ecology of the Russian Federation in 2013. URL: http://government.ru/dep_news/11859/ (reference date 02/04/2016). (In Russ.).

Vetchinnikova L.V., Titov A.F., Kuznetsova T.Y. Karel'skaja bereza: biologiceskie osobennosti, dinamika resursov i vosproizvodstvo [Karelian birch: biological features, dynamics and reproduction of resources]. Petrozavodsk, Karelian Research Centre of Russian Academy of Science, 2013. 312 p. (In Russ.).

Zietkiewicz E. Rafalski A., Labuda D. Genome fingerprinting by simple sequence repeat (SSR) -anchored polymerase chain reaction amplification. Genomics, 1994, V. 20, pp. 176-183.