НАКОПЛЕНИЕ ПОЛИГИДРОКСИАЛКАНОАТОВ В КЛЕТКАХ РОДОКОККОВ ПРИ НЕСБАЛАНСИРОВАННОМ РОСТЕ

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Юлия/Yuliya Геннадьевна/Gennad'evna Максимова/Maksimova
Елена/Elena Юрьевна/Yur'evna Бурлуцкая/Burlutskaya

Аннотация

Определено общее содержание полигидроксиалканоатов (ПГА) в клетках штаммов бактерий рода Rhodococcus при периодическом двустадийном росте на полноценной и лимитированной по источнику азота или фосфора среде культивирования с разными источниками углерода. Методом фазо-во-контрастной световой микроскопии изучена морфология бактериальных клеток при несбалансированном росте. Не было обнаружено значимых различий в накоплении этих запасных питательных веществ как при росте изученных штаммов на разных источниках углерода, так и на средах, лимитированных по азоту или фосфору. Показано, что R. ruber П5-8 накапливает наибольшее количество ПГА на среде, лимитированной по фосфору, при росте на бутирате и ацетате натрия в качестве источника углерода - 312.5 и 466.7 мкг/мг соответственно. При росте R. ruber П5-8 на среде, дефицитной по азоту, отмечается заметное изменение морфологии, которое выражается в неравномерных утолщениях клеток. Наибольшее накопление биомассы R. ruber П5-8 (до 9.4 мг АСБ/мл) наблюдается при росте на среде, лимитированной по фосфору, с бутиратом натрия в качестве источника углерода.

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Как цитировать
Максимова/Maksimova Ю. Г., & Бурлуцкая/Burlutskaya Е. Ю. (2018). НАКОПЛЕНИЕ ПОЛИГИДРОКСИАЛКАНОАТОВ В КЛЕТКАХ РОДОКОККОВ ПРИ НЕСБАЛАНСИРОВАННОМ РОСТЕ. Вестник Пермского университета. Серия Биология, (4), 344–350. извлечено от http://press.psu.ru/index.php/bio/article/view/1771
Раздел
Микробиология
Биографии авторов

Юлия/Yuliya Геннадьевна/Gennad'evna Максимова/Maksimova, ФГБУН Институт экологии и генетики микроорганизмов УрО РАН

Доктор биологических наук, старший научный сотрудник лаборатории молекулярной микробиологии и биотехнологии

Елена/Elena Юрьевна/Yur'evna Бурлуцкая/Burlutskaya, ФГБОУВО «Пермский государственный национальный исследовательский университет»

Аспирант

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